PLC-gamma 2 Antibody (1E10C11) - BSA Free

Western Blot: PLC-gamma 2 Antibody (1E10C11) [NBP2-52535] - Analysis using PLCG2 mAb against HEK293 (1) and PLCG2 (AA: 826-985)-hIgGFc transfected HEK293 (2) cell lysate.

Immunohistochemistry-Paraffin: PLC-gamma 2 Antibody (1E10C11) [NBP2-52535] - Immunohistochemical analysis of paraffin-embedded Hela tissues using PLC-gamma 2 mouse mAb with DAB staining.

Western Blot: PLC-gamma 2 Antibody (1E10C11) [NBP2-52535] - Analysis using PLCG2 mAb against human PLCG2 (AA: 826-985) recombinant protein. (Expected MW is 44.6 kDa)

ELISA: PLC-gamma 2 Antibody (1E10C11) [NBP2-52535] - Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng).

• Reactivity: Hu
• Applications: WB, ELISA, IHC, CyTOF-ready
• Clone: 1E10C11
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1 mg/ml

PLC-gamma 2 Antibody (1E10C11) - BSA Free Summary

• Immunogen: Purified recombinant fragment of human PLC-gamma 2 (AA: 826-985) expressed in E. Coli.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: PLCG2
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • ELISA 1:10000
  • Immunohistochemistry 1:200-1:1000
  • Immunohistochemistry-Paraffin 1:200-1:1000
  • Western Blot 1:500-1:2000
• Application Notes: This antibody is Cytof ready.
• Theoretical MW: 147.9 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein G purified

Alternate Names for PLC-gamma 2 Antibody (1E10C11) - BSA Free

• EC 3.1.4.11
• Phosphoinositide phospholipase C-gamma-2
• phospholipase C gamma 2
• phospholipase C, gamma 2 (phosphatidylinositol-specific)
• phospholipase C, gamma 2 (phosphatidylyinositol-specific)
• Phospholipase C-gamma-2,1-phosphatidylinositol-4,5-bisphosphate phosphodiesterase gamma-2
• Phospholipase C-IV
• PLCG2
• PLCgamma 2
• PLC-gamma 2
• PLC-gamma-2
• PLC-IV

Background

Enzymes of the phospholipase C family catalyze the hydrolysis of phospholipids to yield diacylglycerols and water-soluble phosphorylated derivatives of the lipid head groups. The phospholipase C gamma-2 (PLCgammaII) is an enzyme that plays a crucial role in intracellular signal transduction pathways. This enzyme is important because of its role in the generation of second messengers following the hydrolysis of phosphatidylinositol 4,5-bisphosphate (1). PLCgammaII has been implicated in collagen-induced signal transduction in platelets and antigen-dependent signaling in B-lymphocytes. It has been suggested that tyrosine kinases activate PLCgammaII (2). It has also been shown that PLCgammaII was required for receptor activator of NF-kappaB ligand-induced (RANKL-induced) osteoclastogenesis by differentially regulating nuclear factor of activated T cells c1 (NFATc1), activator protein-1 (AP1), and NF-kappaB (3).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: PLCG2