PLC-gamma 1 Antibody (SN06-10)

Western Blot: PLC-gamma 1 Antibody (SN06-10) [NBP2-67661] - Analysis of PLC-gamma 1 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:200,000 dilution was used for 1 hour at room temperature.Positive control: Lane 1: MCF-7 cell lysateLane 2: SH-SY5Y cell lysate

Immunocytochemistry/Immunofluorescence: PLC-gamma 1 Antibody (SN06-10) [NBP2-67661] - Staining PLC ?1 in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunocytochemistry/Immunofluorescence: PLC-gamma 1 Antibody (SN06-10) [NBP2-67661] - Staining PLC ?1 in SHG-44 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Product Discontinued

PLC-gamma 1 Antibody (SN06-10) Summary

• Additional Information: Recombinant Monoclonal Antibody.
• Immunogen: Recombinant protein within Human PLC-gamma 1 aa 690-852 / 1,290. (SwissProt: P19174 Human; SwissProt: Q62077 Mouse; SwissProt: P10686 Rat)
• Localization: Cell projection.
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: PLCG1
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:50-1:200
  • Immunoprecipitation
  • Western Blot 1:500-1:1000

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: TBS (pH7.4), 0.05% BSA, 40% Glycerol
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for PLC-gamma 1 Antibody (SN06-10)

• EC 3.1.4.11
• gamma 1 (formerly subtype 148)
• monophosphatidylinositol phosphodiesterase
• NCKAP3,1-phosphatidylinositol-4,5-bisphosphate phosphodiesterase gamma-1
• phosphatidylinositol phospholipase C
• phosphoinositidase C
• phosphoinositide phospholipase C
• Phosphoinositide phospholipase C-gamma-1
• phospholipase C, gamma 1
• phospholipase C-148
• Phospholipase C-gamma-1
• Phospholipase C-II
• PLC11-phosphatidylinositol-4,5-bisphosphate phosphodiesterase gamma 1
• PLC148
• PLC-148
• PLCG1
• PLCgamma 1
• PLC-gamma 1
• PLC-gamma-1
• PLC-II1-phosphatidyl-D-myo-inositol-4,5-bisphosphate
• PPLCA
• triphosphoinositide phosphodiesterase

Background

Enzymes of the phospholipase C family catalyze the hydrolysis of phospholipids to yield diacylglycerols and water-soluble phosphorylated derivatives of the lipid head groups (1). Phospholipase C gamma 1 (PLC-gamma 1) hydrolyses phosphatidylinositol-4,5-bisphosphate to the second messengers inositol-1,4,5-trisphosphate and diacylglycerol. PLC-gamma 1 also has mitogenic activity upon growth-factor-dependent tyrosine phosphorylation; however, this activity is not dependent on the phospholipase activity of PLC-gamma 1, but requires an SH3 domain (2). Also, results indicate a lipase-independent role of PLC-gamma in the physiological agonist-induced activation of Ca2+ entry (3).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: PLCG1