PLC-gamma 1 Antibody (2C11)

Immunocytochemistry/Immunofluorescence: PLC-gamma 1 Antibody (2C11) [NB100-74517] - Staining of HeLa cells.

Immunohistochemistry-Paraffin: PLC-gamma 1 Antibody (2C11) [NB100-74517] - Normal biopsies of deparaffinized human tonsil tissue.

Immunohistochemistry-Paraffin: PLC-gamma 1 Antibody (2C11) [NB100-74517] - Cancer biopsies of deparaffinized human hepatocarcinoma.

Immunohistochemistry-Paraffin: PLC-gamma 1 Antibody (2C11) [NB100-74517] - Normal biopsies of deparaffinized human liver tissue.

Product Discontinued

PLC-gamma 1 Antibody (2C11) Summary

• Immunogen: Liposomes (prepared in PBS with lipid A, phosphatidylcholine (PC) and cholesterol) containing synthetic di-palmitoyl PtdIns(4,5)P2.
• Specificity: Prolactin-Induced Protein-1 (2C11)
• Isotype: IgM
• Clonality: Monoclonal
• Host: Mouse
• Gene: PLCG1
• Purity: Unpurified

Applications/Dilutions

• Dilutions:
  • ELISA 1:100 - 1:2000
  • Immunocytochemistry/ Immunofluorescence 1:200
  • Immunohistochemistry 1:200
  • Immunohistochemistry-Paraffin 1:200
  • Immunoprecipitation 1:10 - 1:500
  • Western Blot 1:500
• Application Notes: Useful in WB, IP, ELISA, IF. WB: Detects a 67 kDa protein representing NB100-74517.

Reactivity Notes

Mouse reactivity reported in scientific literature (PMID: 11559758). Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Ascites
• Preservative: 0.05% Sodium Azide
• Purity: Unpurified

Alternate Names for PLC-gamma 1 Antibody (2C11)

• EC 3.1.4.11
• gamma 1 (formerly subtype 148)
• monophosphatidylinositol phosphodiesterase
• NCKAP3,1-phosphatidylinositol-4,5-bisphosphate phosphodiesterase gamma-1
• phosphatidylinositol phospholipase C
• phosphoinositidase C
• phosphoinositide phospholipase C
• Phosphoinositide phospholipase C-gamma-1
• phospholipase C, gamma 1
• phospholipase C-148
• Phospholipase C-gamma-1
• Phospholipase C-II
• PLC11-phosphatidylinositol-4,5-bisphosphate phosphodiesterase gamma 1
• PLC148
• PLC-148
• PLCG1
• PLCgamma 1
• PLC-gamma 1
• PLC-gamma-1
• PLC-II1-phosphatidyl-D-myo-inositol-4,5-bisphosphate
• PPLCA
• triphosphoinositide phosphodiesterase

Background

Phosphatidylinositol-4,5-bisphosphate or PIP2 is an important second messenger for a number of signaling processes. When certain extracellular signals are received Phosphilipase C cleaves phosphatidylinositol into DAG and PIP2. PIP2 has been shown to interact with actin structural proteins including profiling, gelsolin, and cofilin, and demonstrates regulatory function of actin polymerization. It has also been shown to regulate vesicular transport by interacting with proteins like ADP-ribosylation factor and phospholipase D. PIP2 has also been shown to significantly affect intracellular calcium concentrations, which also regulate a variety of activities.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: PLCG1
• Entrez:
  • 5335(Human)