PKR [p Thr446] Antibody

Western Blot: PKR [p Thr446] Antibody [NB100-82156] - Analysis of extracts from K562 cells using PKR(Phospho-Thr446) Antibody (Lane 2) and the same antibody preincubated with blocking peptide(Lane1).

• Reactivity: Hu, Mu, Rt
• Applications: WB
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: 1.0 mg/ml

PKR [p Thr446] Antibody Summary

• Description: Novus Biologicals Rabbit PKR [p Thr446] Antibody (NB100-82156) is a polyclonal antibody validated for use in WB. Anti-PKR Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: The antiserum was produced against synthesized phosphopeptide derived from human PKR around the phosphorylation site of threonine 446 (K-R-TP-R-S).
• Modification: p Thr446
• Specificity: Detects endogenous levels of PKR only when phosphorylated at threonine 446.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: EIF2AK2
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Western Blot 1:500-1:2000
• Theoretical MW: 62 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• Control:

  PKR Overexpression Lysate

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS, 50% glycerol, 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for PKR [p Thr446] Antibody

• double stranded RNA activated protein kinase
• EC 2.7.11.1
• eIF-2A protein kinase 2
• EIF2AK1
• EIF2AK2
• eukaryotic translation initiation factor 2-alpha kinase 2P1/eIF-2A protein kinase
• interferon-induced, double-stranded RNA-activated protein kinase
• interferon-inducible double stranded RNA dependent
• interferon-inducible elF2alpha kinase
• Interferon-inducible RNA-dependent protein kinase
• P68 Kinase
• PKR
• PKRp68 kinase
• PPP1R83
• PRKR
• PRKRMGC126524
• Protein Kinase R
• Protein kinase RNA-activated

Background

The protein encoded by this gene is a serine/threonine protein kinase that is activated by autophosphorylation after binding to dsRNA. The activated form of the encoded protein can phosphorylate translation initiation factor EIF2S1, which in turn inhibits protein synthesis. This protein is also activated by manganese ions and heparin. The encoded protein plays an important role in the innate immune response against multiple DNA and RNA viruses.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for PKR Antibody (NB100-82156)(1)

Publication using NB100-82156

• Cho H, Wu M, Zhang L et al. Signaling dynamics of palmitate-induced ER stress responses mediated by ATF4 in HepG2 cells. BMC Syst Biol 2013-01-22 [PMID: 23339444] (WB, Human)

Bioinformatics

• Gene Symbol: EIF2AK2
• Entrez:
  • 5610(Human)
• Uniprot:
  • P19525(Human)