PKR Antibody (S08-2C8)

Western Blot: PKR Antibody (S08-2C8) [NBP3-19517] - Western blot detection of PKR in K562, Hela cell lysates using NBP3-19517 (1:1000 diluted). Predicted band size: 62kDa. Observed band size: 74kDa.

Immunocytochemistry/Immunofluorescence: PKR Antibody (S08-2C8) [NBP3-19517] - Immunofluorescence of PKR (green) in Hela cells using NBP3-19517 at dilution 1/200, and DAPI (blue)

Immunohistochemistry-Paraffin: PKR Antibody (S08-2C8) [NBP3-19517] - Immunohistochemistry of PKR in paraffin-embedded Human Cholangiocarcinoma using NBP3-19517 at dilution 1/2

• Reactivity: Hu
• Applications: WB, ICC/IF, IHC
• Clone: S08-2C8
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: 0.3 mg/ml

PKR Antibody (S08-2C8) Summary

• Description: Novus Biologicals Rabbit PKR Antibody (S08-2C8) (NBP3-19517) is a recombinant monoclonal antibody validated for use in IHC, WB and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Additional Information: Recombinant Monoclonal Antibody
• Immunogen: A synthetic peptide of human PKR (Uniprot # P19525)
• Source: HEK293
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: EIF2AK2
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1/20
  • Immunohistochemistry 1/20
  • Immunohistochemistry-Paraffin
  • Western Blot 1/2000
• Theoretical MW: 74 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.05% BSA
• Preservative: 0.01% Sodium Azide
• Concentration: 0.3 mg/ml
• Purity: Affinity purified

Alternate Names for PKR Antibody (S08-2C8)

• double stranded RNA activated protein kinase
• EC 2.7.11.1
• eIF-2A protein kinase 2
• EIF2AK1
• EIF2AK2
• eukaryotic translation initiation factor 2-alpha kinase 2P1/eIF-2A protein kinase
• interferon-induced, double-stranded RNA-activated protein kinase
• interferon-inducible double stranded RNA dependent
• interferon-inducible elF2alpha kinase
• Interferon-inducible RNA-dependent protein kinase
• P68 Kinase
• PKR
• PKRp68 kinase
• PPP1R83
• PRKR
• PRKRMGC126524
• Protein Kinase R
• Protein kinase RNA-activated

Background

The protein encoded by this gene is a serine/threonine protein kinase that is activated by autophosphorylation after binding to dsRNA. The activated form of the encoded protein can phosphorylate translation initiation factor EIF2S1, which in turn inhibits protein synthesis. This protein is also activated by manganese ions and heparin. The encoded protein plays an important role in the innate immune response against multiple DNA and RNA viruses.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: EIF2AK2