PKR Antibody (6H3A10)

Images

 
Western Blot: PKR Antibody (6H3A10) [NBP2-37242] - Analysis using PKR mAb against (1) HEK293 and (2) PKR (AA: 329-551)-hIgGFc transfected HEK293 cell lysate.
Immunohistochemistry-Paraffin: PKR Antibody (6H3A10) [NBP2-37242] - Analysis of cervical cancer tissues using EIF2AK2 mouse mAb with DAB staining.
Flow Cytometry: PKR Antibody (6H3A10) [NBP2-37242] - Analysis of A431 cells using EIF2AK2 mouse mAb (green) and negative control (red).
Western Blot: PKR Antibody (6H3A10) [NBP2-37242] - Western blot analysis using EIF2AK2 mAb against human EIF2AK2 (AA: 329-551) recombinant protein. (Expected MW is 51.7 kDa)
Western Blot: PKR Antibody (6H3A10) [NBP2-37242] - Analysis using EIF2AK2 mouse mAb against A431 (1), THP-1 (2), MCF-7 (3), PC-12 (4) cell lysate.
Immunohistochemistry-Paraffin: PKR Antibody (6H3A10) [NBP2-37242] - Analysis of rectum cancer tissues using EIF2AK2 mouse mAb with DAB staining.
ELISA: PKR Antibody (6H3A10) [NBP2-37242] - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ELISA, Flow, IHC, IHC-P, CyTOF-ready
Clone
6H3A10
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

PKR Antibody (6H3A10) Summary

Immunogen
Purified recombinant fragment of human EIF2AK2 (AA: 329-551) expressed in E. Coli.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
EIF2AK2
Purity
Protein A or G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500 - 1:2000
  • ELISA 1:10000
  • Flow Cytometry 1:200 - 1:400
  • Immunohistochemistry 1:200 - 1:1000
  • Immunohistochemistry-Paraffin 1:200-1:1000
  • CyTOF-ready
Application Notes
This antibody is Cytof ready.
Theoretical MW
62 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Control
PKR Knockout HeLa Cell Lysate
PKR Knockout A549 Cell Lysate

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.05% Sodium Azide
Purity
Protein A or G purified

Alternate Names for PKR Antibody (6H3A10)

  • double stranded RNA activated protein kinase
  • EC 2.7.11.1
  • eIF-2A protein kinase 2
  • EIF2AK1
  • EIF2AK2
  • eukaryotic translation initiation factor 2-alpha kinase 2P1/eIF-2A protein kinase
  • interferon-induced, double-stranded RNA-activated protein kinase
  • interferon-inducible double stranded RNA dependent
  • interferon-inducible elF2alpha kinase
  • Interferon-inducible RNA-dependent protein kinase
  • P68 Kinase
  • PKR
  • PKRp68 kinase
  • PPP1R83
  • PRKR
  • PRKRMGC126524
  • Protein Kinase R
  • Protein kinase RNA-activated

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Video Protocols

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Control Lysate(s)

Secondary Antibodies

 

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Blogs on PKR.

eIF2alpha - a regulator of global translation in response to cellular stress
Eukaryotic initiation factor 2 (eIF2) regulates global protein translation by binding to Met-tRNA and the 40S ribosome to form the pre-initiation complex. eIF2 is a heterotrimer consisting of alpha, beta, and gamma subunits. The 36kDA eIF2a subuni...  Read full blog post.

PKR - Mediating cellular stress responses through multiple signaling pathways
Protein kinase R (PKR) is an intracellular stress-sensing protein that is able to detect and respond to viral infections. While PKR is able to sense and respond to a variety of signals, dsRNA is a well-characterized ligand. dsRNA produced during v...  Read full blog post.

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Bioinformatics

Gene Symbol EIF2AK2