PKR Antibody (6H3A10) - BSA Free

Images

 
Western Blot: PKR Antibody (6H3A10) [NBP2-37242] - Analysis using PKR mAb against (1) HEK293 and (2) PKR (AA: 329-551)-hIgGFc transfected HEK293 cell lysate.
Immunohistochemistry-Paraffin: PKR Antibody (6H3A10) [NBP2-37242] - Analysis of cervical cancer tissues using EIF2AK2 mouse mAb with DAB staining.
Flow Cytometry: PKR Antibody (6H3A10) [NBP2-37242] - Analysis of A431 cells using EIF2AK2 mouse mAb (green) and negative control (red).
Western Blot: PKR Antibody (6H3A10) [NBP2-37242] - Western blot analysis using EIF2AK2 mAb against human EIF2AK2 (AA: 329-551) recombinant protein. (Expected MW is 51.7 kDa)
Western Blot: PKR Antibody (6H3A10) [NBP2-37242] - Analysis using EIF2AK2 mouse mAb against A431 (1), THP-1 (2), MCF-7 (3), PC-12 (4) cell lysate.
Immunohistochemistry-Paraffin: PKR Antibody (6H3A10) [NBP2-37242] - Analysis of rectum cancer tissues using EIF2AK2 mouse mAb with DAB staining.
ELISA: PKR Antibody (6H3A10) [NBP2-37242] - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ELISA, Flow, IHC, CyTOF-ready
Clone
6H3A10
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Format
BSA Free
Concentration
1 mg/ml

Order Details

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Catalog# & Formulation Size Price

PKR Antibody (6H3A10) - BSA Free Summary

Immunogen
Purified recombinant fragment of human PKR (AA: 329-551) expressed in E. Coli.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
EIF2AK2
Purity
Protein A or G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • CyTOF-ready
  • ELISA 1:10000
  • Flow Cytometry 1:200 - 1:400
  • Immunohistochemistry 1:200 - 1:1000
  • Immunohistochemistry-Paraffin 1:200-1:1000
  • Western Blot 1:500 - 1:2000
Application Notes
This antibody is Cytof ready.
Theoretical MW
62 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Publications
Read Publications using
NBP2-37242 in the following applications:

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.05% Sodium Azide
Concentration
1 mg/ml
Purity
Protein A or G purified

Alternate Names for PKR Antibody (6H3A10) - BSA Free

  • double stranded RNA activated protein kinase
  • EC 2.7.11.1
  • eIF-2A protein kinase 2
  • EIF2AK1
  • EIF2AK2
  • eukaryotic translation initiation factor 2-alpha kinase 2P1/eIF-2A protein kinase
  • interferon-induced, double-stranded RNA-activated protein kinase
  • interferon-inducible double stranded RNA dependent
  • interferon-inducible elF2alpha kinase
  • Interferon-inducible RNA-dependent protein kinase
  • P68 Kinase
  • PKR
  • PKRp68 kinase
  • PPP1R83
  • PRKR
  • PRKRMGC126524
  • Protein Kinase R
  • Protein kinase RNA-activated

Background

Protein kinase R (PKR) is a serine/threonine kinase that is induced by interferon and activated by double-stranded RNA. Upon activation, PKR is autophosphorylated at multiple sites and phosphorylates the

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for PKR Antibody (NBP2-37242)(2)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 1 application: ICC/IF.


Filter By Application
ICC/IF
(1)
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Filter By Species
Human
(1)
All Species
Showing Publications 1 - 2 of 2.
Publications using NBP2-37242 Applications Species
Gomez R, Tomatis C, Scalise M, Thomas P Yellow Fever Virus Infection Alters Mitochondrial Network Dynamics Authorea 2023-01-18 (ICC/IF, Human) ICC/IF Human
Lukhele S, Rabbo DA, Guo M et al. The transcription factor IRF2 drives interferon-mediated CD8+ T cell exhaustion to restrict anti-tumor immunity Immunity 2022-11-01 [PMID: 36370712]

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Product General Protocols

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Video Protocols

WB Video Protocol

FAQs for PKR Antibody (NBP2-37242) (0)

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Secondary Antibodies

 

Isotype Controls

Additional PKR Products

Research Areas for PKR Antibody (NBP2-37242)

Find related products by research area.

Blogs on PKR.

eIF2alpha - a regulator of global translation in response to cellular stress
Eukaryotic initiation factor 2 (eIF2) regulates global protein translation by binding to Met-tRNA and the 40S ribosome to form the pre-initiation complex. eIF2 is a heterotrimer consisting of alpha, beta, and gamma subunits. The 36kDA eIF2a subuni...  Read full blog post.

PKR - Mediating cellular stress responses through multiple signaling pathways
Protein kinase R (PKR) is an intracellular stress-sensing protein that is able to detect and respond to viral infections. While PKR is able to sense and respond to a variety of signals, dsRNA is a well-characterized ligand. dsRNA produced during v...  Read full blog post.

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Bioinformatics

Gene Symbol EIF2AK2