PKN2 Antibody (3A7) - Azide and BSA Free

Immunocytochemistry/Immunofluorescence: PKN2 Antibody (3A7) [H00005586-M01] - Analysis of monoclonal antibody to PKN2 on HeLa cell . Antibody concentration 10 ug/ml.

Sandwich ELISA: PKN2 Antibody (3A7) [H00005586-M01] - Detection limit for recombinant GST tagged PKN2 is approximately 0.1ng/ml as a capture antibody.

• Reactivity: Hu
• Applications: WB, ELISA, ICC/IF
• Clone: 3A7
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free

PKN2 Antibody (3A7) - Azide and BSA Free Summary

• Description: Quality control test: Antibody Reactive Against Recombinant Protein.
• Immunogen: PKN2 (NP_006247, 580 a.a. ~ 679 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. RASSLGEIDESSELRVLDIPGQDSETVFDIQNDRNSILPKSQSEYKPDTPQSGLEYSGIQELEDRRSQQRFQFNLQDFRCCAVLGRGHFGKVLLAEYKNT
• Specificity: PKN2 - protein kinase N2
• Isotype: IgG1 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: PKN2
• Purity: IgG purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunocytochemistry/ Immunofluorescence
  • Sandwich ELISA
  • Western Blot 1:500
• Application Notes: Antibody reactivity against Recombinant Protein with GST tag on ELISA and WB. GST tag alone is used as a negative control.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: In 1x PBS, pH 7.4
• Preservative: No Preservative
• Purity: IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for PKN2 Antibody (3A7) - Azide and BSA Free

• cardiolipin-activated protein kinase Pak2
• EC 2.7.11
• EC 2.7.11.13
• PAK2
• Pak-2
• PKN2
• PRK2
• PRK2MGC150606
• PRKCL2
• PRKCL2PRO2042
• PRO2042
• Protein kinase C-like 2MGC71074
• protein kinase N2
• Protein-kinase C-related kinase 2
• serine/threonine-protein kinase N2

Background

Protein-kinase-C-related kinases (PRKs) are part of the lipid-regulated protein kinases (PKC) which also include liver PAK & PKN. Human PRK1 and PRK2 share structurally similar catalytic domains, but less similar N-terminal regulatory regions suggesting different regulatory domain functions. PRK1 and PRK2, as well as a third member of this family, PRK3, show distinct patterns of expression in adult tissues. Additionally, the serine-threonine kinase PRK2 can be specifically cleaved by caspase-3 (and/or caspase-3-like subfamily members) during apoptosis

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for PKN2 Antibody (H00005586-M01)(1)

Publication using H00005586-M01

• Bamberger C, Martinez-Bartolome S, Montgomery M et al. Deducing the presence of proteins and proteoforms in quantitative proteomics Nat Commun 2018-06-13 [PMID: 29899466] (WB, Human)

Bioinformatics

• Gene Symbol: PKN2
• Entrez:
  • 5586(Human)
• OMIM:
  • 602549(Human)
• Uniprot:
  • NP_006247(Human)