PKC zeta [p Thr560] Antibody (SN0753)

Western Blot: PKC zeta [p Thr560] Antibody (SN0753) [NBP2-67713] - Analysis of PKC zeta [p Thr560] on SK-Br-3 cell lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:200,000 dilution was used for 1 hour at room temperature.

Immunohistochemistry-Paraffin: PKC zeta [p Thr560] Antibody (SN0753) [NBP2-67713] - Analysis of paraffin-embedded mouse brain tissue using anti-Phospho-PKC zeta(T560) antibody. Counter stained with hematoxylin.

Western Blot: PKC zeta [p Thr560] Antibody (SN0753) [NBP2-67713] - Analysis of Phospho-PKC zeta(T560) on mouse eyeball lysates using anti-Phospho-PKC zeta(T560) antibody at 1/1,000 dilution.

Immunohistochemistry-Paraffin: PKC zeta [p Thr560] Antibody (SN0753) [NBP2-67713] - Analysis of paraffin-embedded human breast carcinoma tissue using anti-Phospho-PKC zeta(T560) antibody. Counter stained with hematoxylin.

Product Discontinued

PKC zeta [p Thr560] Antibody (SN0753) Summary

• Additional Information: Recombinant Monoclonal Antibody.
• Immunogen: Synthetic phospho-peptide corresponding to residues surrounding Thr560 of Human PKC zeta aa 531-580 / 592. (SwissProt: Q05513 Human; SwissProt: Q02956 Mouse; SwissProt: P09217 Rat)
• Modification: p Thr560
• Localization: Cell junction, Cytoplasm, Endosome, Membrane.
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: PRKCZ
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:50-1:200
  • Western Blot 1:1000-1:5000

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: TBS (pH7.4), 0.05% BSA, 40% Glycerol
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for PKC zeta [p Thr560] Antibody (SN0753)

• EC 2.7.11
• EC 2.7.11.13
• nPKC-zeta
• PKC zeta
• PKC2
• PKC2PKC-ZETA
• PRKCZ
• protein kinase C zeta type
• protein kinase C, zeta

Background

Protein kinase C (PKC), a class of serine/threonine kinases activated by Ca2+ and/or phospholipids, is involved in a variety of cellular processes such as proliferation, differentiation and secretion. PKC zeta exhibits a constitutive kinase activity which is independent of Ca2+, phosphatidylserine and diacylglycerol. Arachidonic acid alone or a combination of gamma-linolenic acid and phosphatidylserine slightly enhance PKC zeta activity. PKC zeta, in contrast to PKC alpha, is insensitive to PKC inhibitors known to interfere either with the regulatory or the catalytic domain and cannot be activated by phorbol ester treatment of NIH 3T3 cells or insect cells, overexpressing the respective PKC isoenzyme (1). Results have identified PDK-1 as the kinase that phosphorylates and activates PKC zeta in the PI 3-kinase signaling pathway. This phosphorylation and activation of PKC zeta by PDK-1 is enhanced in the presence of Ptdins-3,4-5-P3. PKCs are enzymes that are regulated at the plasma membrane, and a membrane-targeted PKC zeta is constitutively active in the absence of agonist stimulation. The association between PKC zeta and PDK-1 reveals extensive cross-talk between enzymes in the PI 3-kinase signaling pathway. (2)

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: PRKCZ