PKC mu Antibody (JM30-17)

Western Blot: PKC mu Antibody (JM30-17) [NBP2-67066] - Western blot analysis of PKC mu on PC3 lysate using anti-PKC mu antibody at 1/500 dilution.

Western Blot: PKC mu Antibody (JM30-17) [NBP2-67066] - Analysis of PKC mu on different lysates using anti-PKC mu antibody at 1/1,000 dilution. Positive control: Lane1: Hela Lane2: Mouse kidney Lane3: PC12

Product Discontinued

PKC mu Antibody (JM30-17) Summary

• Additional Information: Recombinant Monoclonal Antibody.
• Immunogen: Synthetic peptide within Human PKC mu aa 201-250 / 912. (SwissProt: Q15139 Human; SwissProt: Q62101 Mouse; SwissProt: Q9WTQ1 Rat)
• Localization: Cell membrane, trans-Golgi network, Cytoplasm.
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: PRKD1
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Western Blot 1:500-1:2000

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: TBS (pH7.4), 0.05% BSA, 40% Glycerol
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for PKC mu Antibody (JM30-17)

• EC 2.7.11
• nPKC-D1
• nPKC-mu
• PKCM
• PKC-MU
• PKD1
• PKDEC 2.7.11.13
• PRKCMPKC-mu
• PRKD1
• Protein kinase C mu type
• protein kinase C, mu
• Protein kinase D
• protein kinase D1
• serine/threonine-protein kinase D1

Background

PKC mu is a novel member of the subgroup of atypical protein kinase Cs (PKC). Deduced protein sequence shows strong homology to conserved domains of members of the PKC subfamily. In vitro phorbol ester binding studies and kinase assays with lysates of cells overexpressing PKC mu showed phorbol ester-independent kinase activity, autophosphorylation, and, in normal rat kidney (NRK) cells, predominant phosphorylation of a 30-kDa protein at serine residues. Data suggest a role of PKC mu in signal transduction pathways related to growth control (1). PKC mu is a cytosolic protein, which upon binding to the trans-Golgi network (TGN) regulates the fission of transport carriers specifically destined to the cell surface. (2). Results demonstrate that betagamma subunits of the heterotrimeric G proteins directly activates PKD by interacting with its pleckstrin homology domain (3).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: PRKD1