PKC gamma Antibody [Alexa Fluor® 350]

• Reactivity: Hu, Mu, Rt
• Applications: WB
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Alexa Fluor 350

PKC gamma Antibody [Alexa Fluor® 350] Summary

• Immunogen: E. coli-derived recombinant human PKCγ
  Arg621-Met697
  Accession # P05129
• Specificity: Detects endogenous human, mouse and rat PKC gamma in Western blots.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Western Blot

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for PKC gamma Antibody [Alexa Fluor® 350]

• EC 2.7.11
• EC 2.7.11.13
• MGC57564
• PKC gamma
• PKCC
• PKCCSCA14
• PKCG
• PKC-gamma
• PKCGprotein kinase C gamma type
• PRKCG
• protein kinase C, gamma
• SCA14

Background

PKC gamma (protein kinase C-gamma) is a 78-80 kDa member of the PKC subfamily, AGC Ser/Thr protein kinase family of enzymes. It is a Ca++-activated and phospholipid-dependent neuronal enzyme that catalyzes the phosphorylation of multiple proteins. Human PKC gamma is 697 amino acids (aa) in length. It contains two general regions: a lipid and Ca++-binding regulatory region (aa 35‑150 and 170‑260, respectively), and an ATP-binding catalytic region (aa 351‑614). PDK1-mediated phosphorylation of PKC gamma on Thr514 activates the enzyme. In addition, oxidative stress also mediates activation by inducing interchain disulfide-bond homodimerization. Over aa 621‑697, human PKC gamma is 99% and 95% aa identical to mouse and canine PKC gamma , respectively. PKC gamma is predominantly expressed in brain tissue.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.