PKC gamma Antibody (JG69-32)

Western Blot: PKC gamma Antibody (JG69-32) [NBP2-75621] - Analysis of PKC gamma on A549 cell lysates using anti-PKC gamma antibody at 1/1,000 dilution.

Immunohistochemistry-Paraffin: PKC gamma Antibody (JG69-32) [NBP2-75621] - Analysis of paraffin-embedded rat cerebellum tissue using anti-PKC gamma antibody. Counter stained with hematoxylin.

Immunohistochemistry-Paraffin: PKC gamma Antibody (JG69-32) [NBP2-75621] - Analysis of paraffin-embedded mouse cerebellum tissue using anti-PKC gamma antibody. Counter stained with hematoxylin.

Product Discontinued

PKC gamma Antibody (JG69-32) Summary

• Additional Information: Recombinant Monoclonal Antibody.
• Immunogen: Recombinant protein within Human PKC gamma aa 564-697 / 697. (SwissProt: P05129 Human; SwissProt: P63318 Mouse; SwissProt: P63319 Rat)
• Localization: Cell membrane. Cytoplasm.
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: PRKCG
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:50-1:200
  • Western Blot 1:500-1:2000
• Theoretical MW: 78 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: TBS (pH7.4), 0.05% BSA, 40% Glycerol
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for PKC gamma Antibody (JG69-32)

• EC 2.7.11
• EC 2.7.11.13
• MGC57564
• PKC gamma
• PKCC
• PKCCSCA14
• PKCG
• PKC-gamma
• PKCGprotein kinase C gamma type
• PRKCG
• protein kinase C, gamma
• SCA14

Background

Protein Kinase C gamma (PKC gamma) is an 80 kDa member of the conventional group (cPKCs: sensitive to calcium, diacylglycerol, phosphotidylserine and phorbol esters) of the PKC family of serine/threonine kinases that are involved in a wide range of physiological processes including mitogenesis, cell survival and transcriptional regulation. PKC gamma plays a key role in neuronal signal transduction and in regulating intercellular communication. The activation loop threonine (threonine 514 in PKC gamma) of conventional PKCs is phosphorylated by phosphoinositide-dependent kinase-1 (PDK1), which is necessary for its autophosphorylation on threonine 655 in the turn loop, and threonine 674 in the hydrophobic loop of the carboxy terminus, a critical step in generating a catalytically mature enzyme. The phosphorylation of the hydrophobic loop in the carboxyl terminus of PKCs is believed to be a key determinant in regulating PKC interaction with PDK1.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: PRKCG