PKC epsilon Antibody (666843) [Unconjugated]

Western blot shows lysates of Human brain (hippocampus) tissue and human brain (hypothalamus) tissue. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human PKCe Monoclonal Antibody (Catalog # MAB51341) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for PKCe at approximately 90 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Simple Western shows lysates of human hippocampus, loaded at 0.5 mg/ml. A specific band was detected for PKC epsilon at approximately 98 kDa (as indicated) using 5 µg/mL of Mouse Anti-Human PKC epsilon Monoclonal Antibody (Catalog # MAB51341). This experiment was conducted under reducing conditions and using the 12-230kDa separation system.

• Reactivity: Hu
• Applications: WB, Simple Western
• Clone: 666843
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated

PKC epsilon Antibody (666843) [Unconjugated] Summary

• Immunogen: E. coli-derived recombinant human PKC epsilon
  Gln580-Pro737
  Accession # Q02156
• Specificity: Detects human PKC epsilon in direct ELISAs and Western blots.
• Source: N/A
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Simple Western 5 ug/mL
  • Western Blot 2 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. See Certificate of Analysis for details.
  *Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for PKC epsilon Antibody (666843) [Unconjugated]

• EC 2.7.11
• EC 2.7.11.13
• MGC125656
• nPKC-epsilon
• PKC epsilon
• PKCE
• PKCEMGC125657
• PRKCE
• protein kinase C epsilon type
• protein kinase C, epsilon

Background

PKC epsilon (protein kinase C-epsilon) is an 87 kDa member of the novel PKC subfamily, AGC Ser/Thr protein kinase family of enzymes. It is a widely-expressed Ca⁺⁺‑insensitive, phospholipid-dependent enzyme that catalyzes the phosphorylation of multiple proteins. Human PKC epsilon is 737 amino acids (aa) in length. It contains two general regions: a non-Ca⁺⁺-binding plus lipid binding regulatory region (aa 1-99 and 169-292, respectively), and an ATP-binding catalytic domain (aa 408-668). Phosphorylation of PKC epsilon on Thr566, Ser368 and Ser729 activates the enzyme and increases its molecular weight to 92 kDa. One potential splice form shows a 114 aa substitution for aa 118-737. Over aa 580-737, human PKC epsilon is 98% and 99% aa identical to mouse and canine PKC epsilon , respectively.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Uniprot:
  • Q02156()