PKC delta Antibody (R20-F) [FITC]

Flow Cytometry: PKC delta Antibody (R20-F) [FITC] [NBP2-21815] - Profile of peripheral blood lymphocytes. Cells were fixed, permeabilized and stained with anti-human PKC delta FITC (blue, used 10 ul per test) or with an isotype control (black).

Product Discontinued

PKC delta Antibody (R20-F) [FITC] Summary

• Additional Information: Recombinant Monoclonal Antibody.
• Immunogen: This antibody targets PKC delta.
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: PRKCD
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1:50-1:100

Packaging, Storage & Formulations

• Storage: Store at 4C in the dark. Do not freeze.
• Buffer: 20mM Tris-HCl (pH 8.0) and 10 mg/ml BSA
• Preservative: 0.05% Sodium Azide
• Purity: Immunogen affinity purified

Notes

This antibody is immunoaffinity purified with immunogenic peptide as a ligand.

Alternate Names for PKC delta Antibody (R20-F) [FITC]

• EC 2.7.11
• EC 2.7.11.13
• MAY1
• MGC49908
• nPKC-delta
• PKC delta
• PKCD
• PKCy
• PRKCD
• protein kinase C delta type
• protein kinase C delta VIII
• protein kinase C, delta

Background

PKC-delta has a biological activity which is closely related to Pkc1, and PKC-delta activates the Pkc1-mediated pathway through an activation of the Bck1 kinase. PKC-delta appears to play a critical role in growth control of yeast and mammalian cells. Suppression experiments suggest that PKC-delta desensitizes the pathway by regulating an aspect of G protein function (1). PKC-delta phosphorylates hRad9 in vitro and in cells exposed to genotoxic agents. It has been shown that PKC-delta is required for binding of hRad9 to Bcl-2. In concert with these results, inhibition of PKC-delta attenuates Rad9-mediated apoptosis. These findings demonstrate that PKC-delta is responsible for the regulation of Rad9 in the Hus1-Rad1 complex and in the apoptotic response to DNA damage (2). It has been reported a mechanism for the regulation of peripheral B-cell survival by serine/threonine protein kinase Cdelta (PKC-delta): spontaneous death of resting B cells is regulated by nuclear localization of PKC-delta that contributes to phosphorylation of histone H2B at serine 14 (S14-H2B) (3).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: PRKCD