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PKC delta Antibody (JJ093-02)

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Western Blot: PKC delta Antibody (JJ093-02) [NBP2-67912] - Analysis of PKC delta on different lysates using anti-PKC delta antibody at 1/1,000 dilution. Positive control: Lane 1: Hela Lane 2: NIH/3T3
Western Blot: PKC delta Antibody (JJ093-02) [NBP2-67912] - Analysis of PKC delta on different lysates using anti-PKC delta antibody at 1/1,000 dilution. Positive control: Lane 1: Hela Lane 2: NIH/3T3
Immunocytochemistry/ Immunofluorescence: PKC delta Antibody (JJ093-02) [NBP2-67912] - Staining PKC delta in RH-35 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, ...read more
Immunohistochemistry-Paraffin: PKC delta Antibody (JJ093-02) [NBP2-67912] - Immunohistochemical analysis of paraffin-embedded mouse liver tissue using anti-PKC delta antibody. The section was pre-treated using heat ...read more
Immunocytochemistry/ Immunofluorescence: PKC delta Antibody (JJ093-02) [NBP2-67912] - Staining PKC delta in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, ...read more
Immunocytochemistry/ Immunofluorescence: PKC delta Antibody (JJ093-02) [NBP2-67912] - Staining PKC delta in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, ...read more
Immunocytochemistry/ Immunofluorescence: PKC delta Antibody (JJ093-02) [NBP2-67912] - Staining PKC delta in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, ...read more
Immunohistochemistry-Paraffin: PKC delta Antibody (JJ093-02) [NBP2-67912] - Analysis of paraffin-embedded human breast carcinoma tissue using anti-PKC delta antibody. Counter stained with hematoxylin.
Immunohistochemistry-Paraffin: PKC delta Antibody (JJ093-02) [NBP2-67912] - Analysis of paraffin-embedded human liver tissue using anti-PKC delta antibody. Counter stained with hematoxylin.
Immunohistochemistry-Paraffin: PKC delta Antibody (JJ093-02) [NBP2-67912] - Analysis of paraffin-embedded human spleen tissue using anti-PKC delta antibody. Counter stained with hematoxylin.
Immunohistochemistry-Paraffin: PKC delta Antibody (JJ093-02) [NBP2-67912] - Analysis of paraffin-embedded human tonsil tissue using anti-PKC delta antibody. Counter stained with hematoxylin.
Immunohistochemistry-Paraffin: PKC delta Antibody (JJ093-02) [NBP2-67912] - Analysis of paraffin-embedded mouse testis tissue using anti-PKC delta antibody. Counter stained with hematoxylin.
Immunohistochemistry-Paraffin: PKC delta Antibody (JJ093-02) [NBP2-67912] - Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-PKC delta antibody. The section was pre-treated using heat ...read more

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-P
Clone
JJ093-02
Clonality
Monoclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1 mg/ml

Order Details

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PKC delta Antibody (JJ093-02) Summary

Additional Information
Recombinant Monoclonal Antibody.
Immunogen
Recombinant protein within mouse PKC delta aa 530-674. (SwissProt: Q05655 Human ;SwissProt: P28867 Mouse ;SwissProt: P09215 Rat)
Localization
Cytoplasm, Nucleus, Cell membrane.
Isotype
IgG
Clonality
Monoclonal
Host
Rabbit
Gene
PRKCD
Purity
Protein A purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • Immunocytochemistry/Immunofluorescence 1:50-1:200
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:50-1:200
  • Western Blot 1:500-1:2000

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
TBS (pH7.4), 1%BSA, 40% Glycerol
Preservative
0.05% Sodium Azide
Concentration
1 mg/ml
Purity
Protein A purified

Alternate Names for PKC delta Antibody (JJ093-02)

  • EC 2.7.11
  • EC 2.7.11.13
  • MAY1
  • MGC49908
  • nPKC-delta
  • PKC delta
  • PKCD
  • PKCy
  • PRKCD
  • protein kinase C delta type
  • protein kinase C delta VIII
  • protein kinase C, delta

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Flow Cytometry: PKC epsilon Antibody (4G8) [NBP2-03620] - Analysis of Hela cells, using anti-PKC epsilon antibody, (Red), compared to a nonspecific negative control antibody (Blue).
Western Blot: PKC epsilon Antibody (4G8) [NBP2-03620] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PKC epsilon (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PKC epsilon.
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Flow Cytometry: Caspase-3 Antibody (31A1067) - (Pro and Active) [NB100-56708] - An intracellular stain was performed on HeLa cells with Caspase-3 Antibody (31A1067) - (Pro and Active) NB100-56708 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature, followed by mouse F(ab)2 IgG (H+L) APC-conjugated secondary antibody (F0101B, R&D Systems).
Flow Cytometry: Caspase-3 Antibody (31A1067) - (Pro and Active) [NB100-56708] - An intracellular stain was performed on NIH3T3 cells with Caspase-3 Antibody (31A1067) - (Pro and Active) Antibody NB100-56708AF700 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 10 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 700.
Western Blot: Caspase-3 Antibody (31A1067) - (Pro and Active) [NB100-56708] - Paclitaxel in combination with MWE retarded tumor growth in a human bladder carcinoma TSGH 8301 xenograft model. The levels of total (t-PTEN) and phospho-PTEN (p-PTEN) and Caspase 3 in the tumor specimens were determined by Western blotting and then quantified using beta-actin as the protein loading control; the results are expressed as a percentage of the control. Image collected and cropped by CiteAb from the following publication (http://www.nature.com/articles/srep20417), licensed under a CC-BY licence.
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Flow Cytometry: ERK2 Antibody (SZ25-01) [NBP2-67360] - Analysis of Hela cells with ERK2 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.
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Immunocytochemistry/Immunofluorescence: PYK2/FAK2 Antibody (SC06-15) [NBP2-67627] - Staining PYK2 in SHG-44 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X-100/PBS.
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Immunocytochemistry/Immunofluorescence: c-jun [p Ser63] Antibody (SY0297) [NBP2-67471] - Staining Phospho-c-Jun(S63) in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
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Western Blot: Cytochrome c Antibody (7H8.2C12) [NB100-56503] - Metformin activates apoptotic cell death; Right panel: MCF-7 cells were kept in the presence or absence of metformin for 20 days and then processed to obtain mitochondrial or whole cellular extracts. BAX and CYCS expression levels were determined by Western blot as indicated under Material and Methods. Densitometric analysis of the gels was performed as indicated under Material and Methods. PHB and CDK4 were used as purity and loading controls. Image collected and cropped by CiteAb from the following publication (http://www.mdpi.com/2073-4409/8/1/49), licensed under a CC-BY licence.
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NFkB p105/p50
Antibody (2J10D7) - B ...
NB100-56583
Flow Cytometry: NFkB p105/p50 Antibody (2J10D7) [NB100-56583] - An intracellular stain was performed on Jurkat cells with NFkB p105/p50 Antibody (2J10D7) NB100-56583C (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes. Both antibodies were conjugated to Dylight 650.
Flow Cytometry: NFkB p105/p50 Antibody (2J10D7) [NB100-56583] - An intracellular stain was performed on HeLa cells with NFkB p105/p50 Antibody (2J10D7) NB100-56583C (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes. Both antibodies were conjugated to Dylight 650.
Flow Cytometry: NFkB p105/p50 Antibody (2J10D7) [NB100-56583] - An intracellular stain was performed on U2-OS cells with NFkB p105/p50 [2J10D7] Antibody NB100-56583C (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to DyLight 650.
(1 Review)
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PKC delta Antibody
(JJ093-02)
NBP2-67912
PKC delta Antibody (JJ093-02)
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P

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Bioinformatics

Gene Symbol PRKCD