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PKC delta Antibody (JJ093-02)

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Western Blot: PKC delta Antibody (JJ093-02) [NBP2-67912] - Analysis of PKC delta on different lysates using anti-PKC delta antibody at 1/1,000 dilution. Positive control: Lane 1: Hela Lane 2: NIH/3T3
Western Blot: PKC delta Antibody (JJ093-02) [NBP2-67912] - Analysis of PKC delta on different lysates using anti-PKC delta antibody at 1/1,000 dilution. Positive control: Lane 1: Hela Lane 2: NIH/3T3
Immunocytochemistry/ Immunofluorescence: PKC delta Antibody (JJ093-02) [NBP2-67912] - Staining PKC delta in RH-35 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, ...read more
Immunohistochemistry-Paraffin: PKC delta Antibody (JJ093-02) [NBP2-67912] - Analysis of paraffin-embedded mouse testis tissue using anti-PKC delta antibody. Counter stained with hematoxylin.
Immunocytochemistry/ Immunofluorescence: PKC delta Antibody (JJ093-02) [NBP2-67912] - Staining PKC delta in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, ...read more
Immunocytochemistry/ Immunofluorescence: PKC delta Antibody (JJ093-02) [NBP2-67912] - Staining PKC delta in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, ...read more
Immunocytochemistry/ Immunofluorescence: PKC delta Antibody (JJ093-02) [NBP2-67912] - Staining PKC delta in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, ...read more
Immunohistochemistry-Paraffin: PKC delta Antibody (JJ093-02) [NBP2-67912] - Analysis of paraffin-embedded human breast carcinoma tissue using anti-PKC delta antibody. Counter stained with hematoxylin.
Immunohistochemistry-Paraffin: PKC delta Antibody (JJ093-02) [NBP2-67912] - Analysis of paraffin-embedded human liver tissue using anti-PKC delta antibody. Counter stained with hematoxylin.
Immunohistochemistry-Paraffin: PKC delta Antibody (JJ093-02) [NBP2-67912] - Analysis of paraffin-embedded human spleen tissue using anti-PKC delta antibody. Counter stained with hematoxylin.
Immunohistochemistry-Paraffin: PKC delta Antibody (JJ093-02) [NBP2-67912] - Analysis of paraffin-embedded human tonsil tissue using anti-PKC delta antibody. Counter stained with hematoxylin.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-P
Clone
JJ093-02
Clonality
Monoclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

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PKC delta Antibody (JJ093-02) Summary

Additional Information
Recombinant Monoclonal Antibody.
Immunogen
Mouse PKC delta aa 500 to the C-terminus
Localization
Cytoplasm, Nucleus, Cell membrane.
Isotype
IgG
Clonality
Monoclonal
Host
Rabbit
Gene
PRKCD
Purity
Protein A purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • Western Blot 1:1000-1:2000
  • Immunocytochemistry/Immunofluorescence 1:50-1:200
  • Immunohistochemistry 1:50-1:200
  • Immunohistochemistry-Paraffin 1:50-1:200
Control
PKC delta Knockout 293T Cell Lysate

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
TBS (pH7.4), 1%BSA, 40%Glycerol
Preservative
0.05% Sodium Azide
Purity
Protein A purified

Alternate Names for PKC delta Antibody (JJ093-02)

  • EC 2.7.11
  • EC 2.7.11.13
  • MAY1
  • MGC49908
  • nPKC-delta
  • PKC delta
  • PKCD
  • PKCy
  • PRKCD
  • protein kinase C delta type
  • protein kinase C delta VIII
  • protein kinase C, delta

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Western Blot: PKC epsilon Antibody (JA38-21) [NBP2-67063] - Analysis of PKC epsilon on different cell lysates using anti-PKC epsilon antibody at 1/1,000 dilution. Positive control:Lane1: Jurkat Lane2: MCF-7 Lane3: HT-29
Immunocytochemistry/Immunofluorescence: PKC epsilon Antibody (JA38-21) [NBP2-67063] - Staining PKC epsilon in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Immunohistochemistry-Paraffin: PKC epsilon Antibody (JA38-21) [NBP2-67063] - Analysis of paraffin-embedded rat brain tissue using anti-PKC epsilon antibody. Counter stained with hematoxylin.
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Western Blot: Caspase-3 Antibody (31A1067) - (Pro and Active) [NB100-56708] - Image of anti-Caspase 3 (31A1067). Whole cell protein from Jurkat cells treated with and without 2 uM staurosporine as indicated was separated on a 4-15% gel by SDS-PAGE, transferred to 0.2 um PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 5 ug/ml anti-Caspase 3 in 1% milk, and detected with an anti-mouse HRP secondary antibody using a Femto sensitivity chemiluminescence reagent. Note the detection of both pro-caspase 3 at 35 kDa and the cleaved active caspase 3 at 15-17 kDa.
Western Blot: Caspase-3 Antibody (31A1067) - (Pro and Active) [NB100-56708] - Paclitaxel in combination with MWE retarded tumor growth in a human bladder carcinoma TSGH 8301 xenograft model. The levels of total (t-PTEN) and phospho-PTEN (p-PTEN) and Caspase 3 in the tumor specimens were determined by Western blotting and then quantified using beta-actin as the protein loading control; the results are expressed as a percentage of the control. Image collected and cropped by CiteAb from the following publication (http://www.nature.com/articles/srep20417), licensed under a CC-BY licence.
Immunohistochemistry-Paraffin: Caspase-3 Antibody (31A1067) - (Pro and Active) [NB100-56708] - Tissue section of human spleen using 1:200 dilution of Caspase-3 antibody (clone 31A1067). The staining was developed with HRP labeled anti-mouse IgG secondary antibody and DAB reagent, and nuclei of cells were counter-stained with hematoxylin. This Caspase 3 antibody generated primarily a specific cytoplasmic staining in a subset of spleenocytes with some nuclear signal in a few cells.
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Immunocytochemistry/Immunofluorescence: ERK2 Antibody (SZ25-01) [NBP2-67360] - Staining ERK2 in NIH/3T3 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
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Immunocytochemistry/Immunofluorescence: ERK1 Antibody (1E5) [NBP2-22203] - Analysis of NIH/3T3 cells using ERK1 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
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Western Blot: AKT1 [p Ser473] Antibody (104A282) [NB100-56749] - WB of phospho AKT using phospho AKT antibody at 2 ug/mL against untreated (lane 1) and PDGF treated (lane 2) NIH-3T3 lysate. HRP conjugated secondary antibody and ECL substrate solution were used for this test. Image using the Azide and BSA Free form of this antibody.
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Immunohistochemistry-Paraffin: c-jun [p Ser63] Antibody (SY0297) [NBP2-67471] - Analysis of paraffin-embedded human tonsil tissue using anti-Phospho-c-Jun(S63) antibody. Counter stained with hematoxylin.
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Western Blot: Cytochrome c Antibody (7H8.2C12) [NB100-56503] - Analysis using the biotin conjugate of NB100-56503. Detection of Cytochrome C in 15 mg of HeLa cell lysate using NB100-55775 at 1:1000. A 15 kDa band is detected.
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Western Blot: NFkB p105/p50 Antibody (2J10D7) [NB100-56583] - Analysis of p50 in HeLa lysate in the A) absence and B) presence of immunizing peptide using p50 antibody at 5 ug/ml.
Immunohistochemistry-Paraffin: NFkB p105/p50 Antibody (2J10D7) [NB100-56583] - IHC-P of rabbit aorta using NB100-56583. Secondary antibody: anti-mouse histofine ( Nisherei Bioscience Inc. ref: 414131F). Development: DAB (Dako ref: K346811-2) and counterstained with Hematoxylin. Submitted via verified customer review
Flow Cytometry: NFkB p105/p50 Antibody (2J10D7) [NB100-56583] - An intracellular stain was performed on U937 cells with NFkB p105/p50 [2J10D7] Antibody NB100-56583C (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to DyLight 650.
(1 Review)
(1 Publication)
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Applications: WB, Flow, IHC, IHC-P, KO
PKC delta Antibody
(JJ093-02)
NBP2-67912
PKC delta Antibody (JJ093-02)
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P

Publications for PKC delta Antibody (NBP2-67912) (0)

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WB Video Protocol
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Control Lysate(s)

PKC delta Knockout 293T Cell Lysate

Secondary Antibodies

 

Isotype Controls

Other Available Formats

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Bioinformatics

Gene Symbol PRKCD
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