PKC beta Antibody (ST48-06)

Western Blot: PKC beta Antibody (ST48-06) [NBP2-67516] - Analysis of PKC beta 2 on Raji cell lysates using anti-PKC beta 2 antibody at 1/1,000 dilution.

Immunocytochemistry/Immunofluorescence: PKC beta Antibody (ST48-06) [NBP2-67516] - Staining PKC beta 2 in SH-SY-5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunohistochemistry-Paraffin: PKC beta Antibody (ST48-06) [NBP2-67516] - Analysis of paraffin-embedded mouse spleen tissue using anti-PKC beta 2 antibody. Counter stained with hematoxylin.

Flow Cytometry: PKC beta Antibody (ST48-06) [NBP2-67516] - Analysis of Hela cells with PKC beta 2 antibody at 1/50 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody?

Immunohistochemistry-Paraffin: PKC beta Antibody (ST48-06) [NBP2-67516] - Analysis of paraffin-embedded human spleen tissue using anti-PKC beta 2 antibody. Counter stained with hematoxylin.

Immunohistochemistry-Paraffin: PKC beta Antibody (ST48-06) [NBP2-67516] - Analysis of paraffin-embedded mouse brain tissue using anti-PKC beta 2 antibody. Counter stained with hematoxylin.

Product Discontinued

PKC beta Antibody (ST48-06) Summary

• Additional Information: Recombinant Monoclonal Antibody.
• Immunogen: Synthetic peptide within Human PKC beta aa 615-660 / 671. (SwissProt: P05771 Human; SwissProt: P68404 Mouse; SwissProt: P68403 Rat)
• Localization: Nucleus, Cytoplasm, Membrane.
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: PRKCB
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1:50-1:100
  • Immunocytochemistry/ Immunofluorescence 1:50-1:200
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:50-1:200
  • Immunoprecipitation
  • Western Blot 1:1000-1:2000

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: TBS (pH7.4), 0.05% BSA, 40% Glycerol
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for PKC beta Antibody (ST48-06)

• EC 2.7.11
• EC 2.7.11.13
• MGC41878
• PKC-B
• PKC-beta
• PKCBPRKCB2
• PRKCB1protein kinase C beta 1
• protein kinase C beta type
• protein kinase C, beta 1 polypeptide
• protein kinase C, beta 1
• protein kinase C, beta

Background

PKCbetaII is a member of the serine/threonine protein kinase C (PKC) family (1) that includes 10 closely related isozymes classified as the

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: PRKCB