PKC beta Antibody (A10-F)

Images

 
Western Blot: PKC beta Antibody (A10-F) [NBP1-30122] - Lane 1 - 50 ng ;Lane 2 - 100 ng; Lane 3 - 200 ng of human recombinant PKC-beta I; MW=76.8kDa.
Immunocytochemistry/ Immunofluorescence: PKC beta Antibody (A10-F) [NBP1-30122] - Expression in HEK293 cells.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ELISA, ICC/IF, IP
Clone
A10-F
Clonality
Monoclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

PKC beta Antibody (A10-F) Summary

Immunogen
Peptide derived from C-terminal sequence of human PKC-beta. Antibody recognizes the epitope located between Ala658 - Glu666.
Specificity
This antibody is specific for PKC Beta 1 variant.
Isotype
IgG
Clonality
Monoclonal
Host
Rabbit
Gene
PRKCB
Purity
Protein A or G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:5000
  • ELISA 1:20000-1:50000
  • Immunocytochemistry/Immunofluorescence 1:150
  • Immunoprecipitation 1:10-1:500
Publications
Read Publication using
NBP1-30122 in the following applications:

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
20mM Tris/HCl (pH 8.0) and 10 mg/ml BSA
Preservative
0.05% Sodium Azide
Purity
Protein A or G purified

Alternate Names for PKC beta Antibody (A10-F)

  • EC 2.7.11
  • EC 2.7.11.13
  • MGC41878
  • PKC-B
  • PKC-beta
  • PKCBPRKCB2
  • PRKCB1protein kinase C beta 1
  • protein kinase C beta type
  • protein kinase C, beta 1 polypeptide
  • protein kinase C, beta 1
  • protein kinase C, beta

Background

Protein kinase C (PKC) beta is implicated as a potential important factor in cancer cell proliferation and angiogenesis. PKC is a family of serine- and threonine-specific protein kinases that can be activated by calcium and second messenger diacylglycerol. PKC family members phosphorylate a wide variety of protein targets and are known to be involved in diverse cellular signaling pathways. PKC family members also serve as major receptors for phorbol esters, a class of tumor promoters. Each member of the PKC family has a specific expression profile and is believed to play a distinct role in cells. The protein encoded by this gene is one of the PKC family members. This protein kinase has been reported to be involved in many different cellular functions, such as B cell activation, apoptosis induction, endothelial cell proliferation, and intestinal sugar absorption. Studies in mice also suggest that this kinase may also regulate neuronal functions and correlate fear-induced conflict behavior after stress. Alternatively spliced transcript variants encoding distinct isoforms have been reported.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for PKC beta Antibody (NBP1-30122)(1)

We have publications tested in 1 confirmed species: Mouse.

We have publications tested in 2 applications: ICC/IF, WB.


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ICC/IF
(1)
WB
(1)
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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Bioinformatics

Gene Symbol PRKCB
Entrez
Uniprot