PKC beta Antibody (A10-F)

Western Blot: PKC beta Antibody (A10-F) [NBP1-30122] - Lane 1 - 50 ng ;Lane 2 - 100 ng; Lane 3 - 200 ng of human recombinant PKC-beta I; MW=76.8kDa.

Immunocytochemistry/Immunofluorescence: PKC beta Antibody (A10-F) [NBP1-30122] - Expression in HEK293 cells.

Product Discontinued

PKC beta Antibody (A10-F) Summary

• Description: This antibody is immunoaffinity purified with immunogenic peptide as a ligand.
• Additional Information: Recombinant Monoclonal Antibody.
• Immunogen: Peptide derived from C-terminal sequence of human PKC beta. Antibody recognizes the epitope located between Ala658 - Glu666.
• Epitope: Ala658 - Glu666
• Specificity: This antibody is specific for PKC Beta 1 variant.
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: PRKCB
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:20000-1:50000
  • Immunocytochemistry/ Immunofluorescence 1:150
  • Immunoprecipitation 1:10-1:500
  • Western Blot 1:5000

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 20mM Tris/HCl (pH 8.0) and 10 mg/ml BSA
• Preservative: 0.05% Sodium Azide
• Purity: Immunogen affinity purified

Notes

This antibody is immunoaffinity purified with immunogenic peptide as a ligand.

Alternate Names for PKC beta Antibody (A10-F)

• EC 2.7.11
• EC 2.7.11.13
• MGC41878
• PKC-B
• PKC-beta
• PKCBPRKCB2
• PRKCB1protein kinase C beta 1
• protein kinase C beta type
• protein kinase C, beta 1 polypeptide
• protein kinase C, beta 1
• protein kinase C, beta

Background

Protein kinase C (PKC) beta is implicated as a potential important factor in cancer cell proliferation and angiogenesis. PKC is a family of serine- and threonine-specific protein kinases that can be activated by calcium and second messenger diacylglycerol. PKC family members phosphorylate a wide variety of protein targets and are known to be involved in diverse cellular signaling pathways. PKC family members also serve as major receptors for phorbol esters, a class of tumor promoters. Each member of the PKC family has a specific expression profile and is believed to play a distinct role in cells. The protein encoded by this gene is one of the PKC family members. This protein kinase has been reported to be involved in many different cellular functions, such as B cell activation, apoptosis induction, endothelial cell proliferation, and intestinal sugar absorption. Studies in mice also suggest that this kinase may also regulate neuronal functions and correlate fear-induced conflict behavior after stress. Alternatively spliced transcript variants encoding distinct isoforms have been reported.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for PKC beta Antibody (NBP1-30122)(1)

Publication using NBP1-30122

• Naik E, Dixit VM. Usp9X Is Required for Lymphocyte Activation and Homeostasis through Its Control of ZAP70 Ubiquitination and PKCbeta Kinase Activity. J. Immunol. 2016-03-02 [PMID: 26936881] (WB, ICC/IF, Mouse)
  
  Details:
  GPR78 antibody was used for WB analysis of lysates from skin fibroblasts of Homocystinuria Patients with Remethylation Defects and control subjects. WB was also performed on lysates from cells treated or not with tunicamycin (Fig 1).

Bioinformatics

• Gene Symbol: PRKCB
• Entrez:
  • 5579(Human)
• Uniprot:
  • P05771()