PKC beta 2 Antibody (469832) [Alexa Fluor® 750]

• Applications: WB
• Clone: 469832
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Alexa Fluor 750

PKC beta 2 Antibody (469832) [Alexa Fluor® 750] Summary

• Immunogen: E. coli-derived recombinant human PKCβ2
  Lys607-Ser673
  Accession # P05771-2
• Specificity: Detects endogenous human and mouse PKC beta 2 in Western blots.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Purity Statement: Protein A or G purified

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Background

Members of the Protein Kinase C (PKC) family are serine/threonine protein kinases that play a key regulatory role in a number of cellular functions including cell growth and differentiation, hormone secretion, and gene expression. Multiple genes and alternative splicing result in three subfamilies, which differ in their co‑factor requirements: conventional PKC isoforms ( alpha , beta Ι, beta ΙΙ, and gamma ) which require calcium and phosphatidylserine (PS), diacylglycerol (DAG) or phorbol esters for activation; novel isoforms (δ, epsilon , eta , and theta ), which are calcium-independent but are still regulated by PS, DAG, or phorbol esters; and atypical isoforms ( iota / lambda , and zeta ), which are calcium-independent and do not require PS, DAG, or phorbol esters for activation. PKC beta 2 regulation of c-myc expression has been shown to suppress insulin gene transcription in pancreatic beta -cells implicating PKC beta 2 for some of the beta -cell glucose toxicity found in diabetes.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.