PKA C beta Antibody (2G8-1D12) - Azide and BSA Free

Sandwich ELISA: PKA C beta Antibody (2G8-1D12) [H00005567-M01] - Detection limit for recombinant GST tagged PRKACB is approximately 1ng/ml as a capture antibody.

• Reactivity: Hu
• Applications: ELISA
• Clone: 2G8-1D12
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free

PKA C beta Antibody (2G8-1D12) - Azide and BSA Free Summary

• Description: Quality control test: Antibody Reactive Against Recombinant Protein.
• Immunogen: PRKACB (AAH16285, 1 a.a. ~ 257 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MGNAATAKKGSEVESVKEFLAKAKEDFLKKWENPTQNNAGLEDFERKKTLGTGSFGRVMLVKHKATEQYYAMKILDKQKVVKLKQIEHTLNEKRILQAVNFPFLVRLEYAFKDNSNLYMVMEYVPGGEMFSHLRRIGRFSEPHARFYAAQIVLTFEYLHSLDLIYRDLKPENLLIDHQGYIQVTDFGFAKRVKGRTWTLCGTPEYLAPEIILSKGYNKAVDWWALGVLIYEMAAGYPPFFADQPIQIYEKIVSGKNF
• Localization: Inactive holoenzyme, monomeric catalytic subunit: Cytoplasm. Monomeric catalytic subunit: Nucleus. Translocates into the nucleus (monomeric).
• Specificity: PRKACB - protein kinase, cAMP-dependent, catalytic, beta
• Isotype: IgG1 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: PRKACB
• Purity: IgG purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Sandwich ELISA
• Application Notes: Antibody reactivity against recombinant protein on ELISA. GST alone used as a negative control.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: In 1x PBS, pH 7.4
• Preservative: No Preservative
• Purity: IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for PKA C beta Antibody (2G8-1D12) - Azide and BSA Free

• cAMP-dependent protein kinase catalytic beta subunit isoform 4ab
• cAMP-dependent protein kinase catalytic subunit beta
• cAPKbeta
• DKFZp781I2452
• EC 2.7.11
• EC 2.7.11.11
• MGC41879
• MGC9320
• PKA C beta
• PKA C-beta
• PKACb
• PRKACB
• protein kinase A catalytic subunit beta
• protein kinase, cAMP-dependent, catalytic, beta

Background

cAMP is a signaling molecule important for a variety of cellular functions. cAMP exerts its effects by activating the cAMP-dependent protein kinase, which transduces the signal through phosphorylation of different target proteins. The inactive kinase holoenzyme is a tetramer composed of two regulatory and two catalytic subunits. cAMP causes the dissociation of the inactive holoenzyme into a dimer of regulatory subunits bound to four cAMP and two free monomeric catalytic subunits. Four different regulatory subunits and three catalytic subunits have been identified in humans. The protein encoded by this gene is a member of the Ser/Thr protein kinase family and is a catalytic subunit of cAMP-dependent protein kinase. Three alternatively spliced transcript variants encoding distinct isoforms have been observed.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: PRKACB
• Entrez:
  • 5567(Human)
• OMIM:
  • 176892(Human)
• Uniprot:
  • AAH16285(Human)