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Recombinant Human PKA C alpha Protein

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SDS-Page: Recombinant Human PKA C alpha Protein [NBP2-59647] - 15% SDS-PAGE (3ug)
SDS-Page: Recombinant Human PKA C alpha Protein [NBP2-59647] - 15% SDS-PAGE (3ug)

Product Details

Summary
Reactivity HuSpecies Glossary
Applications PAGE
Concentration
0.25 mg/ml

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Recombinant Human PKA C alpha Protein Summary

Description
A recombinant protein with a N-Terminal GST-tag and corresponding to the amino acids 1-351 of Human PKA C alpha

Source: Baculovirus

Amino Acid Sequence: MSPILGYWKI KGLVQPTRLL LEYLEEKYEE HLYERDEGDK WRNKKFELGL EFPNLPYYID GDVKLTQSMA IIRYIADKHN MLGGCPKERA EISMLEGAVL DIRYGVSRIA YSKDFETLKV DFLSKLPEML KMFEDRLCHK TYLNGDHVTH PDFMLYDALD VVLYMDPMCL DAFPKLVCFK KRIEAIPQID KYLKSSKYIA WPLQGWQATF GGGDHPPKSD LVPRGSHMGN AAAAKKGSEQ ESVKEFLAKA KEDFLKKWES PAQNTAHLDQ FERIKTLGTG SFGRVMLVKH KETGNHYAMK ILDKQKVVKL KQIEHTLNEK RILQAVNFPF LVKLEFSFKD NSNLYMVMEY VPGGEMFSHL RRIGRFSEPH ARFYAAQIVL TFEYLHSLDL IYRDLKPENL LIDQQGYIQV TDFGFAKRVK GRTWTLCGTP EYLAPEIILS KGYNKAVDWW ALGVLIYEMA AGYPPFFADQ PIQIYEKIVS GKVRFPSHFS SDLKDLLRNL LQVDLTKRFG NLKNGVNDIK NHKWFATTDW IAIYQRKVEA PFIPKFKGPG DTSNFDDYEE EEIRVSINEK CGKEFSEF
Source
Baculovirus
Protein/Peptide Type
Recombinant Protein
Gene
PRKACA
Purity
>85%, by SDS-PAGE
Endotoxin Note
< 1.0 EU per 1 microgram of protein (determined by LAL method)

Applications/Dilutions

Theoretical MW
67 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
In PBS (pH 7.4) containing 10% glycerol.
Preservative
No Preservative
Concentration
0.25 mg/ml
Purity
>85%, by SDS-PAGE

Alternate Names for Recombinant Human PKA C alpha Protein

  • cAMP-dependent protein kinase catalytic subunit alpha
  • Cs
  • EC 2.7.11
  • EC 2.7.11.11
  • MGC102831
  • MGC48865
  • PKA C alpha
  • PKA C-alpha
  • Pkaca
  • PKCA1
  • PKCD
  • PRKACA
  • protein kinase A catalytic subunit
  • protein kinase, cAMP-dependent, catalytic, alpha

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

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1 µg/lane of Recombinant Human TNF-alpha was resolved by SDS-PAGE with silver staining, under reducing (R) conditions, showing a band at 17 kDa.
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1 µg/lane of Recombinant Human EGF was resolved&amp;nbsp;with SDS-PAGE&amp;nbsp; and visualized&amp;nbsp;by silver staining under reducing (R) conditions, showing a single band at 6 kDa.
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CFTR was detected in immersion fixed paraffin-embedded sections of human placenta using Mouse&amp;nbsp;Anti-Human CFTR C-Terminus Monoclonal Antibody (Catalog # MAB25031) at 25&amp;nbsp;µg/mL overnight at 4&amp;nbsp;°C. Tissue was stained using the Anti-Mouse HRP-DAB Cell &amp;amp; Tissue Staining Kit (brown; Catalog&amp;nbsp;# CTS002) and counterstained with hematoxylin (blue). Lower panel shows a lack of labeling if primary antibodies are omitted and tissue is stained only with secondary antibody followed by incubation with detection reagents. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
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Bioinformatics

Gene Symbol PRKACA
Entrez