PKA C alpha Antibody

Immunocytochemistry/Immunofluorescence: PKA C alpha Antibody [NBP2-75620] - Staining PKA C-alpha in PC-3M cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunohistochemistry-Paraffin: PKA C alpha Antibody [NBP2-75620] - Analysis of paraffin-embedded rat brain tissue using anti-PKA C-alpha antibody. Counter stained with hematoxylin.

Flow Cytometry: PKA C alpha Antibody [NBP2-75620] - Analysis of PC-3M cells with PKA C-alpha antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black).

Immunocytochemistry/Immunofluorescence: PKA C alpha Antibody [NBP2-75620] - Staining PKA C-alpha in A431 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunohistochemistry-Paraffin: PKA C alpha Antibody [NBP2-75620] - Analysis of paraffin-embedded human kidney tissue using anti-PKA C-alpha antibody. Counter stained with hematoxylin.

Immunohistochemistry-Paraffin: PKA C alpha Antibody [NBP2-75620] - Analysis of paraffin-embedded mouse testis tissue using anti-PKA C-alpha antibody. Counter stained with hematoxylin.

Product Discontinued

PKA C alpha Antibody Summary

• Immunogen: Recombinant protein within human PKA C alpha aa 172-338. (SwissProt: P17612 Human; SwissProt: P05132 Mouse; SwissProt: P27791 Rat)
• Localization: Mitochondrion. Nucleus.Plasma membrane.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: PRKACA
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1:50-1:100
  • Immunocytochemistry/ Immunofluorescence 1:50-1:200
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:50-1:200
• Theoretical MW: 40 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH7.4), 0.2% BSA, 50% Glycerol
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for PKA C alpha Antibody

• cAMP-dependent protein kinase catalytic subunit alpha
• Cs
• EC 2.7.11
• EC 2.7.11.11
• MGC102831
• MGC48865
• PKA C alpha
• PKA C-alpha
• Pkaca
• PKCA1
• PKCD
• PRKACA
• protein kinase A catalytic subunit
• protein kinase, cAMP-dependent, catalytic, alpha

Background

PKA (Protein Kinase A), also known as cAMP-dependent protein kinase, phosphorylates serine or threonine residues in target protein in response to elevated levels of cAMP. Inactive PKA exists as a tetrameric protein composed of two regulatory (R) subunits and two catalytic (C) subunits. Activation occurs when two cAMP molecules bind to each R subunit which causes a conformational change that releases the active C subunits.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: PRKACA