Recombinant Human PIST His Protein

SDS-Page: PIST Protein [NBP1-40404] - GOPC, 21.5 kDa (198aa), confirmed by MALDI-TOF with a purity of 90% by SDS - PAGE

• Reactivity: Hu
• Applications: PAGE
• Concentration: 1 mg/ml

Recombinant Human PIST His Protein Summary

• Description: A recombinant protein with a N-Terminal His-tag and corresponding to the amino acids 278-454 of Human PIST
  
  

  Source: E.coli

  Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MIRKVLLLKE DHEGLGISIT GGKEHGVPIL ISEIHPGQPA DRCGGLHVGD AILAVNGVNL RDTKHKEAVT ILSQQRGEIE FEVVYVAPEV DSDDENVEYE DESGHRYRLY LDELEGGGNP GASCKDTSGE IKVLQGFNKK AVTDTHENGD LGTASETPLD DGASKLDDLH TLYHKKSY

• Source: E. coli
• Protein/Peptide Type: Recombinant Protein
• Gene: GOPC
• Purity: >90%, by SDS-PAGE

Applications/Dilutions

• Dilutions:
  • SDS-Page
• Theoretical MW: 21.5 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 20 mM Tris-HCl buffer (pH 8.0), 10% glycerol, 1 mM DTT
• Preservative: No Preservative
• Concentration: 1 mg/ml
• Purity: >90%, by SDS-PAGE

Alternate Names for Recombinant Human PIST His Protein

• CALPDZ protein interacting specifically with TC10
• CFTR-associated ligand
• dJ94G16.2
• FIGdJ94G16.2 PIST
• Fused in glioblastoma
• golgi-associated PDZ and coiled-coil motif containing
• Golgi-associated PDZ and coiled-coil motif-containing protein
• GOPC1
• PDZ/coiled-coil domain binding partner for the rho-family GTPase TC10
• PISTGolgi associated PDZ and coiled-coil motif containing protein

Background

GOPC, also known as PIST, is a PDZ domain-containing Golgi protein. This protein functions as a homooligomer that interacts with a variety of proteins and plays a role in intracellular protein trafficking and degradation. Additionally, GOPC is thought to regulate ionic currents via membrane channel modification and may also play a role in autophagy. Chromosomal aberrations in the gene encoding GOPC are found in glioblastoma multiform (GBM), a common and aggressive form of brain tumor, suggesting a role for mutated PIST in carcinogenesis. Recombinant GOPC protein was expressed in E.coli and purified by using conventional chromatography techniques.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

Bioinformatics

• Gene Symbol: GOPC