PIST Antibody (JG79-35)

Western Blot: PIST Antibody (JG79-35) [NBP2-75618] - Analysis of PIST on SiHa cell using anti-PIST antibody at 1/1,000 dilution.

Immunocytochemistry/Immunofluorescence: PIST Antibody (JG79-35) [NBP2-75618] - Staining PIST in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunohistochemistry-Paraffin: PIST Antibody (JG79-35) [NBP2-75618] - Analysis of paraffin-embedded mouse brain tissue using anti-PIST antibody. Counter stained with hematoxylin.

Flow Cytometry: PIST Antibody (JG79-35) [NBP2-75618] - Analysis of MCF-7 cells with PIST antibody at 1/100 dilution (purple) compared with an unlabelled control (cells without incubation with primary antibody; yellow). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.

Immunocytochemistry/Immunofluorescence: PIST Antibody (JG79-35) [NBP2-75618] - Staining PIST in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunocytochemistry/Immunofluorescence: PIST Antibody (JG79-35) [NBP2-75618] - Staining PIST in SKOV-3 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunohistochemistry-Paraffin: PIST Antibody (JG79-35) [NBP2-75618] - Analysis of paraffin-embedded human liver cancer tissue using anti-PIST antibody. Counter stained with hematoxylin.

Immunohistochemistry-Paraffin: PIST Antibody (JG79-35) [NBP2-75618] - Analysis of paraffin-embedded human colon tissue using anti-PIST antibody. Counter stained with hematoxylin.

Immunohistochemistry-Paraffin: PIST Antibody (JG79-35) [NBP2-75618] - Analysis of paraffin-embedded human kidney tissue using anti-PIST antibody. Counter stained with hematoxylin.

Product Discontinued

PIST Antibody (JG79-35) Summary

• Additional Information: Recombinant Monoclonal Antibody.
• Immunogen: Recombinant protein within Human PIST aa 1-140 / 462. (SwissProt: Q9HD26 Human; SwissProt: Q8BH60 Mouse)
• Localization: Cytoplasm. Golgi apparatus. Plasma membrane.
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: GOPC
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1:50-1:100
  • Immunocytochemistry/ Immunofluorescence 1:50-1:200
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:50-1:200
  • Immunoprecipitation 1:10-1:50
  • Western Blot 1:500-1:2000
• Theoretical MW: 51 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: TBS (pH7.4), 0.05% BSA, 40% Glycerol
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for PIST Antibody (JG79-35)

• CALPDZ protein interacting specifically with TC10
• CFTR-associated ligand
• dJ94G16.2
• FIGdJ94G16.2 PIST
• Fused in glioblastoma
• golgi-associated PDZ and coiled-coil motif containing
• Golgi-associated PDZ and coiled-coil motif-containing protein
• GOPC1
• PDZ/coiled-coil domain binding partner for the rho-family GTPase TC10
• PISTGolgi associated PDZ and coiled-coil motif containing protein

Background

PIST is a PDZ domain-containing Golgi protein. PDZ domains contain approximately 90 amino acids and bind the extreme Cterminus of proteins in a sequence-specific manner.(supplied by OMIM)

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Review for PIST Antibody (NBP2-75618) (1)

Average Rating: 5

(Based on 1 review)

We have 1 review tested in 1 species: Human.

reviewed by: Teddy Chan WB Human 02/08/2019

Summary

• Application: Western Blot
• Sample Tested: brain vascular smooth muscle cells
• Species: Human
• Lot: HL0502

Bioinformatics

• Gene Symbol: GOPC