PIP5K2 alpha Antibody (OTI3D3) - Azide and BSA Free

Western Blot: PIP5K2 alpha Antibody (OTI3D3) - Azide and BSA Free [NBP2-73417] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PIP5K2 alpha (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PIP5K2 al

Immunohistochemistry: PIP5K2 alpha Antibody (OTI3D3) - Azide and BSA Free [NBP2-73417] - Staining of paraffin-embedded Human lymphoma tissue using anti-PIP5K2 alpha mouse monoclonal antibody.

Flow Cytometry: PIP5K2 alpha Antibody (OTI3D3) - Azide and BSA Free [NBP2-73417] - Analysis of Jurkat cells, using anti-PIP5K2 alpha antibody, (Red), compared to a nonspecific negative control antibody (Blue).

Immunohistochemistry: PIP5K2 alpha Antibody (OTI3D3) - Azide and BSA Free [NBP2-73417] - Staining of paraffin-embedded Human lymph node tissue using anti-PIP5K2 alpha mouse monoclonal antibody.

Flow Cytometry: PIP5K2 alpha Antibody (OTI3D3) - Azide and BSA Free [NBP2-73417] - Analysis of Hela cells, using anti-PIP5K2 alpha antibody, (Red), compared to a nonspecific negative control antibody (Blue).

Flow Cytometry: PIP5K2 alpha Antibody (OTI3D3) - Azide and BSA Free [NBP2-73417] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostaining by anti-PIP5K2 alpha antibody, and then analyzed by flow cytometry.

• Reactivity: Hu, Mu, Rt
• Applications: WB, Flow, IHC, CyTOF-ready
• Clone: OTI3D3
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free
• Concentration: LYOPH

PIP5K2 alpha Antibody (OTI3D3) - Azide and BSA Free Summary

• Immunogen: Full length human recombinant protein of human PIP4K2A (NP_005019) produced in HEK293T cell.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: PIP4K2A
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • Flow Cytometry 1:100
  • Immunohistochemistry 1:150
  • Immunohistochemistry-Paraffin
  • Western Blot 1:200 - 1:1000
• Theoretical MW: 46 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Lyophilized from PBS (pH 7.3) with 8% Trehalose
• Preservative: No Preservative
• Concentration: LYOPH
• Purity: Immunogen affinity purified
• Reconstitution Instructions: we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Alternate Names for PIP5K2 alpha Antibody (OTI3D3) - Azide and BSA Free

• 1-phosphatidylinositol-4-phosphate kinase
• 1-phosphatidylinositol-4-phosphate-5-kinase
• 1-phosphatidylinositol-5-phosphate 4-kinase 2-alpha
• Diphosphoinositide kinase 2-alpha
• EC 2.7.1
• EC 2.7.1.149
• FLJ13267
• phosphatidylinositol-4-phosphate 5-kinase, type II, alpha
• Phosphatidylinositol-5-phosphate 4-kinase type II alpha
• phosphatidylinositol-5-phosphate 4-kinase type-2 alpha
• phosphatidylinositol-5-phosphate 4-kinase, type II, alpha
• PI(5)P 4-kinase type II alpha
• PIP4KII-alpha
• PIP5K2
• PIP5K2API5P4KA
• PIP5KIIA
• PIP5KIIalpha
• PIP5KII-alpha
• PIP5KIII
• PIPK
• PtdIns(4)P-5-kinase B isoform
• PtdIns(4)P-5-kinase C isoform
• PtdIns(5)P-4-kinase isoform 2-alpha
• type II phosphatidylinositol-4-phosphate 5-kinase 53 K isoform

Background

Phosphatidylinositol-5,4-bisphosphate, the precursor to second messengers of the phosphoinositide signal transduction pathways, is thought to be involved in the regulation of secretion, cell proliferation, differentiation, and motility. The protein encoded by this gene is one of a family of enzymes capable of catalyzing the phosphorylation of phosphatidylinositol-5-phosphate on the fourth hydroxyl of the myo-inositol ring to form phosphatidylinositol-5,4-bisphosphate. The amino acid sequence of this enzyme does not show homology to other kinases, but the recombinant protein does exhibit kinase activity. This gene is a member of the phosphatidylinositol-5-phosphate 4-kinase family.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: PIP4K2A