PIM2 Antibody

Western Blot: PIM2 Antibody [NBP2-26115] - HEK293 lysate (10 ug protein in RIPA buffer) overexpressing Human PIM2 with C-terminal MYC tag probed with NBP2-26115 (0.1 ug/ml) in Lane A and probed with anti-MYC Tag (1/1000) in lane C. Mock-transfected HEK293 probed with NBP2-26115 (0.1 mg/ml) in Lane B. Primary incubations were for 1 hour. Detected by chemiluminescence.

Immunohistochemistry-Paraffin: PIM2 Antibody [NBP2-26115] - 5ug/ml staining of paraffin embedded Human Heart. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

• Reactivity: Hu
• Applications: WB, ELISA, IHC
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

PIM2 Antibody Summary

• Description: Novus Biologicals Goat PIM2 Antibody (NBP2-26115) is a polyclonal antibody validated for use in IHC, WB and ELISA. Anti-PIM2 Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Peptide with sequence C-KDREAFEAEYRLGP corresponding to internal region (near N-Terminus) according to NP_006866.2.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: PIM2
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 0.5-1ug/ml
  • Immunohistochemistry-Paraffin
  • Peptide ELISA Detection limit 1:64000
  • Western Blot 1:100 - 1:2000
• Application Notes: Western blot: In transfected HEK293 transiently expressing Human PIM2 (myc and DYKDDDDK tagged), a band of approx. 38kDa is observed. No bands are observed in mock-transfected HEK293 and the same band is observed using anti-myc antibody.
  IHC: Paraffin embedded Human Heart.

Reactivity Notes

Expected from sequence similarity: Mouse, Rat, Bovine

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for PIM2 Antibody

• EC 2.7.11.1
• pim-2 oncogene
• PIM2
• pim-2h
• proto-oncogene Pim-2 (serine threonine kinase)
• serine/threonine protein kinase pim-2
• serine/threonine-protein kinase pim-2

Background

PIM2 belongs to a family of cytoplasmic serine/threonine protein kinases that consists of two other members, PIM1 and PIM3. Expression of this protein is regulated by hematopoietic cytokines. PIM2 is most abundantly expressed in hematopoietic tissues, spleen, thymus, and peripheral blood leukocytes, as well as in testis, small intestine, and colon. It is also highly expressed in human leukemic and lymphoma cell lines. The gene is also expressed in primary spermatocytes. The results suggest a role for PIM2 in proliferating cells as well as during meiosis. PIM2 is thought to be a mediator of p53 mediated apoptosis inhibition by interleukin 3, which supports its role in the formation of various tumours including lymphomas.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for PIM2 Antibody (NBP2-26115)(1)

Publication using NBP2-26115

• Hammerman PS, Fox CJ, Cinalli RM et al. Lymphocyte transformation by Pim-2 is dependent on nuclear factor-kappaBactivation. Cancer Res 2004-11-15 [PMID: 15548703]

Bioinformatics

• Gene Symbol: PIM2
• Entrez:
  • 11040(Human)
  • 18715(Mouse)
  • 317366(Rat)