PIM2 Antibody (447023) [DyLight 550]

• Reactivity: Hu, Mu, Rt
• Applications: WB, ICC/IF
• Clone: 447023
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: DyLight 550

PIM2 Antibody (447023) [DyLight 550] Summary

• Immunogen: E. coli-derived recombinant human PIM2
  Met1-Pro311
  Accession # Q9P1W9
• Specificity: Detects human, mouse, and rat PIM2 in Western blots. In Western blots, no cross-reactivity with recombinant human PIM1 or PIM3 is observed.
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Gene: PIM2
• Purity: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry
  • Western Blot
• Application Notes: Optimal dilution of this antibody should be experimentally determined.

Packaging, Storage & Formulations

• Storage: Store at 4C in the dark.
• Buffer: 50mM Sodium Borate
• Preservative: 0.05% Sodium Azide
• Purity: Protein A or G purified from hybridoma culture supernatant

Notes

DyLight (R) is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries.

Alternate Names for PIM2 Antibody (447023) [DyLight 550]

• EC 2.7.11.1
• pim-2 oncogene
• PIM2
• pim-2h
• proto-oncogene Pim-2 (serine threonine kinase)
• serine/threonine protein kinase pim-2
• serine/threonine-protein kinase pim-2

Background

PIM2 belongs to a family of cytoplasmic serine/threonine protein kinases that consists of two other members, PIM1 and PIM3. Expression of this protein is regulated by hematopoietic cytokines. PIM2 is most abundantly expressed in hematopoietic tissues, spleen, thymus, and peripheral blood leukocytes, as well as in testis, small intestine, and colon. It is also highly expressed in human leukemic and lymphoma cell lines. The gene is also expressed in primary spermatocytes. The results suggest a role for PIM2 in proliferating cells as well as during meiosis. PIM2 is thought to be a mediator of p53 mediated apoptosis inhibition by interleukin 3, which supports its role in the formation of various tumours including lymphomas.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: PIM2