pIgR Antibody (825724) [Unconjugated]

Western blot shows lysates of human stomach tissue. PVDF membrane was probed with 2.0 µg/mL of Mouse Anti-Human pIgR Monoclonal Antibody (Catalog # MAB2717) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for pIgR at approximately 85-100 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Simple Western lane view shows lysates of human stomach tissue, loaded at 1 mg/mL. A specific band was detected for pIgR at approximately 110-150 kDa (as indicated) using 2.5 µg/mL of Mouse Anti-Human pIgR Monoclonal Antibody (Catalog # MAB2717). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

• Reactivity: Hu
• Applications: WB, Simple Western
• Clone: 825724
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: LYOPH

pIgR Antibody (825724) [Unconjugated] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant human pIgR
  Lys19-Arg638
  Accession # CAA51532
• Specificity: Detects human pIgR in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant mouse pIgR is observed.
• Source: N/A
• Isotype: IgG3
• Clonality: Monoclonal
• Host: Mouse
• Gene: PIGR
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Simple Western 2.5 ug/mL
  • Western Blot 2.0 ug/mL
• Application Notes: In Simple Western only 10-15 uL of the recommended dilution is used per data point.

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. See Certificate of Analysis for details.
  *Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for pIgR Antibody (825724) [Unconjugated]

• FLJ22667
• hepatocellular carcinoma associated protein TB6
• Hepatocellular carcinoma-associated protein TB6
• MGC125361
• MGC125362
• pIgR
• Poly-Ig receptor
• polymeric immunoglobulin receptor

Background

The human polymeric immunoglobulin receptor (pIgR; also known as membrane secretory component) is an approximately 100 kDa type I transmembrane glycoprotein that is synthesized as a 764 amino acid (aa) precursor. It includes a signal sequence (aa 1-18), an extracellular region (aa 19-638), a transmembrane segment (aa 639-661), and a cytoplasmic domain (aa 662-764) (1-3). The extracellular region consists of five Ig-like domains and a sixth non-Ig domain that connects to the membrane region. pIgR is expressed on secretory epithelial cells of exocrine tissues. Immunoglobulin isotypes consist of two heavy (H) and two light (L) chains. For IgA and IgM, this H₂L₂ monomer can form larger polymers through association with a joining chain (J chain). The Fc regions of IgA and IgM have a carboxy-terminal extension called a secretory tailpiece that binds the J chain (4). pIgR functions as a carrier that transports IgA and IgM across epithelium (5). On the basolateral surface of epithelial cells, the receptor initially binds non-covalently to IgA via a docking site on the J chain. This initiates a rearrangement in which a disulfide bond forms between pIgR and an IgA heavy chain (2). The complexes are then internalized and transcytosed to the apical surface. A soluble covalent complex called secretory IgA (SIgA) is now generated by proteolytic cleavage of the sixth extracellular domain of pIgR and released into the lumen (6). This IgA-bound and proteolytically generated pIgR fragment is referred to as secretory component (SC). Notably, human pIgR transcytoses constitutively, with or without ligand, creating both bound and free, 78 kDa SC following cleavage (3). The extracellular region of pIgR shares 64%, 65%, and 70% aa sequence identity with the equivalent region of rat, mouse and porcine pIgR, respectively. The receptor component of the complex anchors the SIgA molecule to mucous (7). SIgA is a crucial component of the mucosal immune system serving to protect the large expanse of mucous membranes that form a barrier between the interior of the body and the external environment (8).

1. Krajci, P. et al. (1989) Biochem. Biophys. Res. Commun. 158:783.
2. Piskurich, J. et al. (1995) J. Immunol. 154:1735.
3. Brandtzaeg, P. and F-E. Johansen (2001) Trends Immunol. 22:545.
4. Braathen, R. et al. (2002) J. Biol. Chem. 277:42755.
5. Ben-Hur, H. et al. (2004) Int. J. Mol. Med. 14:35.
6. Asano, M. et al. (2004) Immunology 112:583.
7. Phalipon, A. and B. Corthesy (2003) Trends Immunol. 24:55.
8. Uren, T. et al. (2003) J. Immunol. 170:2531.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for pIgR Antibody (MAB2717)(1)

Publication using MAB2717

• Iovino , Federico, Molema , Grietje, Bijlsma , Jetta J Streptococcus pneumoniae Interacts with pIgR expressed by the brain microvascular endothelium but does not co-localize with PAF receptor. PLoS ONE, 2014-05-19;9(5):e97914. 2014-05-19 [PMID: 24841255] (Western Blot, Human)

Bioinformatics

• Gene Symbol: PIGR
• Uniprot:
  • CAA51532()