PIG3 Antibody (1C9) - BSA Free

Western Blot: PIG3 Antibody (1C9) [NBP2-22591] - The Cell lysates (1ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human PIG3 antibody (1:3000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1.: 293T cell lysate Lane 2.: PIG3 Transfected 293T cell lysate

Immunocytochemistry/Immunofluorescence: PIG3 Antibody (1C9) [NBP2-22591] - ICC/IF analysis of PIG3 in A549 cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human PIG3 antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).

Flow Cytometry: PIG3 Antibody (1C9) [NBP2-22591] - Flow cytometry analysis of PIG3 in A549 cell line, staining at 2-5ug for 1x106cells (red line). The secondary antibody used goat anti-mouse IgG Alexa fluor 488 conjugate. Isotype control antibody was mouse IgG (black line).

Western Blot: PIG3 Antibody (1C9) [NBP2-22591] - The Cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human PIG3 antibody (1:3000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1. : A549 cell lysate Lane 2. : SW480 cell lysate

• Reactivity: Hu
• Applications: WB, ELISA, ICC/IF
• Clone: 1C9
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1 mg/ml

PIG3 Antibody (1C9) - BSA Free Summary

• Description: Novus Biologicals Mouse PIG3 Antibody (1C9) - BSA Free (NBP2-22591) is a monoclonal antibody validated for use in WB, ELISA and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Recombinant human PIG3 (1-332aa) purified from E. coli
• Isotype: IgG2a Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: TP53I3
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:100-1:2000
  • Immunocytochemistry/ Immunofluorescence 1:100
  • Western Blot 1:3000

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.4), 10% Glycerol
• Preservative: 0.02% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein G purified

Alternate Names for PIG3 Antibody (1C9) - BSA Free

• p53-induced gene 3 protein
• PIG3quinone oxidoreductase homolog
• quinone oxidoreductase PIG3
• tumor protein p53 inducible protein 3
• Tumor protein p53-inducible protein 3

Background

TP53I3 participates in the generation of reactive oxygen species (ROS). TP53I3 has low NADPH-dependent naphtoquinone reductase activity, with a preference for 1,2-naphtoquinone over 1,4-naphtoquinone. TP53I3 has low NADPH-dependent diamine reductase activity (in vitro). TP53I3 is localized to the cytoplasm and induced in primary, non-transformed and transformed cell cultures after exposure to genotoxic agents. TP53I3 microsatellite polymorphism is associated with differential susceptibility to cancer.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: TP53I3
• Uniprot:
  • NP_671713(Human)