Piccolo Antibody (6H9-B6)

Images

 
Western Blot: Piccolo Antibody (6H9-B6) [NBP1-49453] - analysis of Rat and mouse brain lysates showing detection of Piccolo protein using Mouse Anti-Piccolo Monoclonal Antibody, Clone 6H9-B6. Primary Antibody: Mouse ...read more
Immunocytochemistry/ Immunofluorescence: Piccolo Antibody (6H9-B6) [NBP1-49453] - Analysis using Mouse Anti-Piccolo Monoclonal Antibody, Clone 6H9-B6. Tissue: Neuroblastoma cell line SK-N-BE. Species: Human. Fixation: ...read more

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ELISA, ICC/IF
Clone
6H9-B6
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1 mg/ml

Piccolo Antibody (6H9-B6) Summary

Immunogen
Full length protein, BC001304.1. No cross-reactivity to Bassoon, band at approx. 550kDa corresponding to the molecular weight of Piccolo.
Localization
Cell Junction, Synapse
Marker
Synaptic Marker
Specificity
Detects approx 550kDa.
Isotype
IgG2b Kappa
Clonality
Monoclonal
Host
Mouse
Gene
PCLO
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:2000
  • ELISA 1 ug/ml
  • Immunocytochemistry/Immunofluorescence
Application Notes
1 ug/ml of Piccolo Antibody was sufficient for detection of Piccolo in 20 ug of rat brain tissue lysate by colorimetric immunoblot analysis using goat IgG:HRP as the secondary Antibody.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.4) and 50% Glycerol
Preservative
0.09% Sodium Azide
Concentration
1 mg/ml
Purity
Protein G purified

Alternate Names for Piccolo Antibody (6H9-B6)

  • ACZ
  • Aczonin
  • PCLO
  • piccolo (presynaptic cytomatrix protein)
  • Piccolo
  • protein piccolo

Background

Piccolo is a presynaptic cytomatrix protein concentrated at the presynaptic side of synaptic junctions. Piccolo is a large protein which constists of an N-terminal Zn2+ finger, several piccolo-bassoon homology domains (PBH-domains) and C-terminal PDZ and C2 domains. It is usually found together with bassoon, a related huge multi-domain protein of the CAZ (cytoskeletal matric assembled at active zones). Piccolo is a scaffolding protein for proteins involved in endo- and exocytosis of synaptic vesicles. Recently piccolo has been shown to interfere with clathrin mediated endocytosis by binding to the F-actin and dynamin binding protein Abp1. Piccolo is highly expressed in brain with low levels found in stomach. It is not detected in other tissues analyzed including adrenal gland, testis and pancreas.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol PCLO
Entrez
Uniprot