Piccolo Antibody (6H9-B6) - BSA Free

Western Blot: Piccolo Antibody (6H9-B6) [NBP1-49453] - Western Blot analysis of Rat and mouse brain lysates showing detection of Piccolo protein using Mouse Anti-Piccolo Monoclonal Antibody, Clone 6H9-B6 (NBP1-49453). Primary Antibody: Mouse Anti-Piccolo Monoclonal Antibody (NBP1-49453) at 1:1000. (1) Mouse brain lysate; (2) Rat brain lysate.

Immunocytochemistry/Immunofluorescence: Piccolo Antibody (6H9-B6) [NBP1-49453] - Analysis using Mouse Anti-Piccolo Monoclonal Antibody, Clone 6H9-B6. Tissue: Neuroblastoma cell line SK-N-BE. Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-Piccolo Monoclonal Antibody at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60min RT, 5min RT. Localization: Cytoplasm, Endoplasmic Reticulum, Nucleus. Magnification: 60X. (A) DAPI (blue) nuclear stain (B) Phalloidin Texas Red F-Actin stain (C) Piccolo Antibody (D) Composite.

Western Blot: Piccolo Antibody (6H9-B6) [NBP1-49453] - analysis of Rat and mouse brain lysates showing detection of Piccolo protein using Mouse Anti-Piccolo Monoclonal Antibody, Clone 6H9-B6 . Primary Antibody: Mouse Anti-Piccolo Monoclonal Antibody at 1:1000. (1) Mouse brain lysate; (2) Rat brain lysate.

• Reactivity: Hu, Mu, Rt
• Applications: WB, ELISA, ICC/IF
• Clone: 6H9-B6
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1 mg/ml

Piccolo Antibody (6H9-B6) - BSA Free Summary

• Description: Novus Biologicals Mouse Piccolo Antibody (6H9-B6) - BSA Free (NBP1-49453) is a monoclonal antibody validated for use in WB, ELISA and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Full length protein, BC001304.1
• Localization: Cell Junction , Synapse
• Marker: Synaptic Marker
• Isotype: IgG2b Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: PCLO
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunocytochemistry/ Immunofluorescence 1:100
  • Western Blot 1:1000
• Application Notes: 1 ug/ml of Piccolo Antibody was sufficient for detection of Piccolo in 20 ug of rat brain tissue lysate by colorimetric immunoblot analysis using goat IgG:HRP as the secondary Antibody.
• Theoretical MW: 550 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

No cross-reactivity to Bassoon.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.4), 50% Glycerol
• Preservative: 0.09% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein G purified

Alternate Names for Piccolo Antibody (6H9-B6) - BSA Free

• ACZ
• Aczonin
• PCLO
• piccolo (presynaptic cytomatrix protein)
• Piccolo
• protein piccolo

Background

Piccolo is a presynaptic cytomatrix protein concentrated at the presynaptic side of synaptic junctions. Piccolo is a large protein which constists of an N-terminal Zn2+ finger, several piccolo-bassoon homology domains (PBH-domains) and C-terminal PDZ and C2 domains. It is usually found together with bassoon, a related huge multi-domain protein of the CAZ (cytoskeletal matric assembled at active zones). Piccolo is a scaffolding protein for proteins involved in endo- and exocytosis of synaptic vesicles. Recently piccolo has been shown to interfere with clathrin mediated endocytosis by binding to the F-actin and dynamin binding protein Abp1. Piccolo is highly expressed in brain with low levels found in stomach. It is not detected in other tissues analyzed including adrenal gland, testis and pancreas.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: PCLO
• Entrez:
  • 27445(Human)
• Uniprot:
  • BC001304.1(Human)