Piccolo Antibody (4G3.3) - BSA Free

Western Blot: Piccolo Antibody (4G3.3) [NBP2-29749] - Rat brain lysate was resolved by electrophoresis, transferred to PVDF membrane and probed with ant- Piccolo, clone 4G3.3

Immunohistochemistry: Piccolo Antibody (4G3.3) [NBP2-29749] - Immunohistochemistry (Paraffin): Representative images from a previous lot. Piccolo staining pattern/morphology on mouse brain tissue/cerebellum. Tissue was pretreated with citrate pH 6.0, antigen retrieval. A previous lot of the antibody was diluted to 1:300, using IHC-Select Detection with HRP-DAB. Immunoreactivity is localized to the synaptic junction of neurons in the granular layer and Purkinje cells(arrow).

• Reactivity: Mu, Rt
• Applications: WB, IHC
• Clone: 4G3.3
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1 mg/ml

Piccolo Antibody (4G3.3) - BSA Free Summary

• Description: Relevant Entrez Gene Numbers: NP_001104266
• Immunogen: GST-tagged recombinant protein.
• Marker: Synaptic Marker
• Specificity: This antibody recognizes piccolo.
• Isotype: IgM Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: PCLO
• Purity: Ammonium sulfate precipitation

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:10-1:500
  • Western Blot 1:100-1:2000
• Application Notes: Immunohistochemistry-Paraffin:Representative images from a previous lot.Piccolo staining pattern/morphology on mouse brain tissue/cerebellum. Tissue was pretreated with citrate pH 6.0, antigen retrieval. A previous lot of the antibody was diluted to 1:300, using IHC-Select Detection with HRP-DAB. Immunoreactivity is localized to the synaptic junction of neurons in the granular layer and Purkinje cells(arrow).
• Theoretical MW: 547 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: PBS
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Ammonium sulfate precipitation

Notes

Relevant Entrez Gene Numbers: NP_001104266

Alternate Names for Piccolo Antibody (4G3.3) - BSA Free

• ACZ
• Aczonin
• PCLO
• piccolo (presynaptic cytomatrix protein)
• Piccolo
• protein piccolo

Background

Piccolo (presynaptic cytomatrix protein or PCLO) may act as a scaffolding protein involved in the organization of synaptic active zones. Synaptic vesicles dock and fuse in the active zone of the plasma membrane at chemical synapses. The presynaptic cytoskeletal matrix (PCM), which is associated with the active zone and is situated between synaptic vesicles, is thought to be involved in maintaining the neurotransmitter release site in register with the postsynaptic reception apparatus. The cycling of synaptic vesicles is a multistep process involving a number of proteins. Among the components of the PCM that orchestrate these events are Bassoon (BSN), RIM (RBBP8), Oboe, and Piccolo (PCLO) [supplied by ENTREZGENE].

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: PCLO
• Uniprot:
  • Q9Y6V0(Human)