Human PIBF1 ELISA Kit (Colorimetric)

ELISA: Human PIBF1 ELISA Kit (Colorimetric) [NBP2-68096] - Samples were spiked with high concentrations of Human PIBF1 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

ELISA: Human PIBF1 ELISA Kit (Colorimetric) [NBP2-68096] - Standard Curve Reference

• Reactivity: Hu
• Applications: ELISA
• Suitable Sample Type: Serum, plasma and other biological fluids
• Standard Curve Range: 0.31 - 20 ng/mL
• Sensitivity: 0.19 ng/mL

Human PIBF1 ELISA Kit (Colorimetric) Summary

• Specificity: This kit recognizes Human PIBF in samples. No significant cross-reactivity or interference between Human PIBF and analogues was observed.
• Standard Curve Range: 0.31 - 20 ng/mL
• Sensitivity: 0.19 ng/mL
• Assay Type: Sandwich-ELISA
• Inter-Assay: CV% < 5.32%
• Intra-Assay: CV% < 5.26%
• Spike Recovery: 93-107%
• Sample Volume: 100 uL
• Kit Type: ELISA Kit (Colorimetric)
• Gene: PIBF1

Applications/Dilutions

• Dilutions:
  • ELISA

Packaging, Storage & Formulations

• Storage: Storage of components varies. See protocol for specific instructions.

Alternate Names for Human PIBF1 ELISA Kit (Colorimetric)

• C13orf24
• chromosome 13 open reading frame 24
• KIAA1008
• PIBF
• PIBF1
• progesterone immunomodulatory binding factor 1
• progesterone-induced blocking factor 1
• progesterone-induced-blocking factor 1
• RP11-505F3.1

Background

PIBF (progesterone-induced blocking factor 1) is synthesized during pregnancy in response to progesterone by progesterone receptor-positive T lymphocytes (mostly gamma-delta T cells). In the presence of PIBF, natural killer (NK) cells inhibit the release of perforin from storage granules and, therefore, fail to lyse target cells. In humans, the amount of cells that express PIBF is significantly higher in healthy pregnant women than in women at risk for premature pregnancy termination. PIBF has a predicted 89 kDa molecular mass; the full-length PIBF is associated with the nucleus, whereas secretion of shorter forms, such a 34 kDa protein is induced by activation of the cell. Research suggests that PIBF functions as a transcription factor in its full-length form, while smaller forms may act as cytokines.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

Bioinformatics

• Gene Symbol: PIBF1