PI 3-Kinase p85 beta Antibody (T15)

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Product Details

Summary
Reactivity Hu, Mu, Rt, Bv, PmSpecies Glossary
Applications WB, IHC, IHC-Fr, IP
Clone
T15
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1.0 mg/ml

Order Details

PI 3-Kinase p85 beta Antibody (T15) Summary

Immunogen
Balculovirus expressed (Recombinant) Bovine P85 beta subunit of P13 kinase.
Specificity
Recognizes the p85 subunit of bovine PI-3 kinase.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
PIK3R2
Purity
Protein A or G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:100-1:2000
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Frozen 1:10-1:500
  • Immunoprecipitation 1:10-1:500

Reactivity Notes

Reacts with Rat, Human, Monkey, Mouse and Bovine. Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.09% Sodium Azide
Concentration
1.0 mg/ml
Purity
Protein A or G purified

Alternate Names for PI 3-Kinase p85 beta Antibody (T15)

  • P85B
  • p85-beta
  • phosphatidylinositol 3-kinase 85 kDa regulatory subunit beta
  • phosphatidylinositol 3-kinase regulatory subunit beta
  • phosphatidylinositol 3-kinase, regulatory subunit, polypeptide 2 (p85 beta)
  • phosphoinositide-3-kinase, regulatory subunit 2 (beta)
  • phosphoinositide-3-kinase, regulatory subunit 2 (p85 beta)
  • phosphoinositide-3-kinase, regulatory subunit, polypeptide 2 (p85 beta)
  • PI 3Kinase p85 beta
  • PI 3-Kinase p85 beta
  • PI3K regulatory subunit beta
  • PI3-kinase regulatory subunit beta
  • PI3-kinase subunit p85-beta
  • PIK3R2
  • ptdIns-3-kinase regulatory subunit beta
  • ptdIns-3-kinase regulatory subunit p85-beta

Background

The enzyme phosphatidylinositol 3 kinase (PI3 kinase) is a lipid kinase that generates phosphatidylinositol 3, 4, 5-triphosphate in response to receptor activation in many signal transduction pathways. Class IA PI3Ks exist as a heterodimer of a catalytic 110 kDa (p110) and a regulatory p85 subunit (e.g. p85 beta). p85 beta is an adaptor molecule that regulates the activity of the catalytic p110 subunit by binding to phosphorylated receptor tyrosine kinases (RTKs) through its SH2 domain and mediating the interaction between p110 and the plasma membrane. p85 beta is necessary for insulin-stimulated increase in glucose uptake and glycogen synthesis in insulin-sensitive tissues.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Secondary Antibodies

 

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Bioinformatics

Gene Symbol PIK3R2
Entrez
Uniprot