PI 3-Kinase p85 alpha Antibody (3A10)

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Western Blot: PI 3-Kinase p85 alpha Antibody (3A10) [H00005295-M01] - PIK3R1 monoclonal antibody (M01), clone 3A10. Analysis of PIK3R1 expression in NIH/3T3.
Western Blot: PI 3-Kinase p85 alpha Antibody (3A10) [H00005295-M01] - PIK3R1 monoclonal antibody (M01), clone 3A10 Analysis of PIK3R1 expression in HeLa.
Western Blot: PI 3-Kinase p85 alpha Antibody (3A10) [H00005295-M01] - PIK3R1 monoclonal antibody (M01), clone 3A10. Analysis of PIK3R1 expression in PC-12.
Western Blot: PI 3-Kinase p85 alpha Antibody (3A10) [H00005295-M01] - PIK3R1 monoclonal antibody (M01), clone 3A10. Analysis of PIK3R1 expression in Raw 264.7.
Western Blot: PI 3-Kinase p85 alpha Antibody (3A10) [H00005295-M01] - Analysis of PIK3R1 expression in transfected 293T cell line by PIK3R1 monoclonal antibody (M01), clone 3A10.Lane 1: PIK3R1 transfected lysate ...read more
Proximity Ligation Assay: PI 3-Kinase p85 alpha Antibody (3A10) [H00005295-M01] - Analysis of protein-protein interactions between EGFR and PIK3R1. HeLa cells were stained with anti-EGFR rabbit purified polyclonal ...read more
Proximity Ligation Assay: PI 3-Kinase p85 alpha Antibody (3A10) [H00005295-M01] - Analysis of protein-protein interactions between FLT1 and PIK3R1. Huh7 cells were stained with anti-FLT1 rabbit purified polyclonal ...read more
Proximity Ligation Assay: PI 3-Kinase p85 alpha Antibody (3A10) [H00005295-M01] - Analysis of protein-protein interactions between CRKL and PIK3R1. Mahlavu cells were stained with anti-CRKL rabbit purified polyclonal ...read more
Immunoprecipitation: PI 3-Kinase p85 alpha Antibody (3A10) [H00005295-M01] - Analysis of PIK3R1 transfected lysate using anti-PIK3R1 monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with PIK3R1 ...read more
Sandwich ELISA: PI 3-Kinase p85 alpha Antibody (3A10) [H00005295-M01] - Detection limit for recombinant GST tagged PIK3R1 is approximately 0.1ng/ml as a capture antibody.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ELISA, IP, PLA
Clone
3A10
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

PI 3-Kinase p85 alpha Antibody (3A10) Summary

Immunogen
PIK3R1 (AAH30815, 1 a.a. - 454 a.a.) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MYNTVWNMEDLDLEYAKTDINCGTDLMFYIEMDPPALPPKPPKPTTVANNGMNNNMSLQDAEWYWGDISREEVNEKLRDTADGTFLVRDASTKMHGDYTLTLRKGGNNKLIKIFHRDGKYGFSDPLTFSSVVELINHYRNESLAQYNPKLDVKLLYPVSKYQQDQVVKEDNIEAVGKKLHKYNTQFQEKSREYDRLYEEYTRTSQEIQMKRTAIEAFNETIKIFEEQCQTQERYSKEYIEKFKREGNEKEIQRIMHNYDKLKSRISEIIDSRRRLEEDLKKQAAEYREIDKRMNSIKPDLIQLRKTRDQYLMWLTQKGVRQKKLNEWLGNENTEDQYSLVEDDEDLPHHDEKTWNVGSSNRNKAENLLRGKRDGTFLVRESSKQGCYACSVVVDGEVKHCVINKTATGYGFAEPYNLYSSLKELVLHYQHTSLVQHNDSLNVTLAYPVYAQQRR
Specificity
PIK3R1 - phosphoinositide-3-kinase, regulatory subunit 1 (p85 alpha)
Isotype
IgG1 Kappa
Clonality
Monoclonal
Host
Mouse
Gene
PIK3R1
Purity
IgG purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot
  • ELISA
  • Immunoprecipitation
  • Proximity Ligation Assay
Application Notes
Antibody reactivity against cell lysate and recombinant protein for WB. It has also been used for ELISA.
Publications
Read Publication using H00005295-M01.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.4)
Preservative
No Preservative
Purity
IgG purified

Notes

Quality control test: Antibody Reactive Against Recombinant Protein.

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for PI 3-Kinase p85 alpha Antibody (3A10)

  • GRB1
  • GRB1PI3-kinase subunit p85-alpha
  • p85
  • p85-ALPHA
  • Phosphatidylinositol 3-kinase 85 kDa regulatory subunit alpha
  • phosphatidylinositol 3-kinase regulatory subunit alpha
  • phosphatidylinositol 3-kinase, regulatory subunit, polypeptide 1 (p85 alpha)
  • phosphatidylinositol 3-kinase, regulatory, 1
  • phosphatidylinositol 3-kinase-associated p-85 alpha
  • phosphoinositide-3-kinase, regulatory subunit 1 (alpha)
  • PI 3Kinase p85 alpha
  • PI 3-Kinase p85 alpha
  • PI3K regulatory subunit alpha
  • PI3-kinase regulatory subunit alpha
  • PIK3R1
  • PtdIns-3-kinase regulatory subunit alpha
  • PtdIns-3-kinase regulatory subunit p85-alpha

Background

Phosphatidylinositol 3-kinase phosphorylates the inositol ring of phosphatidylinositol at the 3-prime position. The enzyme comprises a 110 kD catalytic subunit and a regulatory subunit of either 85, 55, or 50 kD. This gene encodes the 85 kD regulatory subunit. Phosphatidylinositol 3-kinase plays an important role in the metabolic actions of insulin, and a mutation in this gene has been associated with insulin resistance. Alternative splicing of this gene results in three transcript variants encoding different isoforms.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for PI 3-Kinase p85 alpha Antibody (H00005295-M01)(1)

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Bioinformatics

Gene Symbol PIK3R1
Entrez
OMIM
Uniprot