PI 3-Kinase p110 gamma Knockout HeLa Cell Lysate

Product Discontinued

PI 3-Kinase p110 gamma Knockout HeLa Cell Lysate Summary

• Preparation Method: Knockout achieved by using CRISPR/Cas9,-16 bp deletion in exon and 1 bp insertion in exon
• Gene: PIK3CG

Applications/Dilutions

• Dilutions:
  • Western Blot
• Application Notes: You will receive 1 vial (100ug) of knockout cell lysate and 1 vial (100ug) of Parental cell lysate. Lysate can be diluted with 1X SDS sample buffer and will be stable at -20 degrees C for 12 months. Minimize freeze-thaw cycles.

Packaging, Storage & Formulations

• Storage: Store at -20C short term. Aliquot and store at -80C long term. Avoid freeze-thaw cycles.
• Buffer: 0.1 mg cell homogenate lyophilized in RIPA buffer made with double-knockout cell lines.
• Concentration: LYOPH
• Reconstitution Instructions: To use as WB negative control, spin down briefly and resuspend in 100 uL 1xSDS sample buffer (2% SDS, 60 mM Tris-HCl pH 6.8, 10% Glycerol, 0.02% Bromophenol blue, 60 mM beta-mercaptoethanol). Boil the lysate for 3 - 5 minutes before loading it onto gel.

Lysate Details for Array

• Type: Knockout HeLa Cell

Notes

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Validation of antibody specificity is critical and verification of antibody performance against knockout samples is one way to guarantee that an antibody recognizes a specific target. Novus' KO cell lysate can be used as a negative control for western blots and to confirm the specificity of antibodies.

Alternate Names for PI 3-Kinase p110 gamma Knockout HeLa Cell Lysate

• 1-phosphatidylinositol 3-kinase
• EC 2.7.1
• EC 2.7.1.153
• p110-gamma
• p120-PI3K
• phosphatidylinositol 3 kinase gamma, p110 gamma
• phosphatidylinositol 3-kinase catalytic 110-kD gamma
• phosphatidylinositol 3-kinase, catalytic, gamma polypeptide
• Phosphatidylinositol-4,5-bisphosphate 3-kinase 110 kDa catalytic subunit gamma
• phosphatidylinositol-4,5-bisphosphate 3-kinase catalytic subunit gamma isoform
• phosphoinositide-3-kinase gamma catalytic subunit
• phosphoinositide-3-kinase, catalytic, gamma polypeptide
• PI 3Kinase p110 gamma
• PI 3-Kinase p110 gamma
• PI3CG
• PI3K
• PI3K-gamma
• PI3Kgamma
• PI3K-gamma
• PI3-kinase subunit gamma
• PIK3
• PIK3CG
• PtdIns-3-kinase subunit gamma
• PtdIns-3-kinase subunit p110-gamma

Background

Phosphoinositide 3-kinases (PI3Ks) phosphorylate inositol lipids and are involved in the immune response. The protein encoded by this gene is a class I catalytic subunit of PI3K. Like other class I catalytic subunits (p110-alpha p110-beta, and p110-delta), the encoded protein binds a p85 regulatory subunit to form PI3K. This gene is located in a commonly deleted segment of chromosome 7 previously identified in myeloid leukemias. Several transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Jun 2015]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

Bioinformatics

• Gene Symbol: PIK3CG