PI 3-Kinase p110 delta Knockout HeLa Cell Lysate

Product Discontinued

PI 3-Kinase p110 delta Knockout HeLa Cell Lysate Summary

• Preparation Method: Knockout achieved by using CRISPR/Cas9
• Gene: PIK3CD

Applications/Dilutions

• Dilutions:
  • Western Blot
• Application Notes: You will receive 1 vial (100ug) of knockout cell lysate and 1 vial (100ug) of Parental cell lysate. Lysate can be diluted with 1X SDS sample buffer and will be stable at -20 degrees C for 12 months. Minimize freeze-thaw cycles.

Packaging, Storage & Formulations

• Storage: Store at -20C short term. Aliquot and store at -80C long term. Avoid freeze-thaw cycles.
• Buffer: 0.1 mg cell homogenate lyophilized in RIPA buffer made with double-knockout cell lines.
• Concentration: LYOPH
• Reconstitution Instructions: To use as WB negative control, spin down briefly and resuspend in 100 uL 1xSDS sample buffer (2% SDS, 60 mM Tris-HCl pH 6.8, 10% Glycerol, 0.02% Bromophenol blue, 60 mM beta-mercaptoethanol). Boil the lysate for 3 - 5 minutes before loading it onto gel.

Lysate Details for Array

• Type: Knockout HeLa Cell

Notes

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Validation of antibody specificity is critical and verification of antibody performance against knockout samples is one way to guarantee that an antibody recognizes a specific target. Novus' KO cell lysate can be used as a negative control for western blots and to confirm the specificity of antibodies.

Alternate Names for PI 3-Kinase p110 delta Knockout HeLa Cell Lysate

• EC 2.7.1
• EC 2.7.1.153
• p110D
• P110DELTA
• Phosphatidylinositol-4,5-bisphosphate 3-kinase 110 kDa catalytic subunit delta
• phosphatidylinositol-4,5-bisphosphate 3-kinase catalytic subunit delta isoform
• phosphoinositide-3-kinase C
• phosphoinositide-3-kinase, catalytic, delta polypeptide
• PI 3Kinase p110 delta
• PI 3-Kinase p110 delta
• PI3K
• PI3K-delta
• PI3-kinase p110 subunit delta
• PI3-kinase subunit delta
• PIK3CD
• PtdIns-3-kinase subunit delta
• PtdIns-3-kinase subunit p110-delta

Background

Phosphoinositide 3-kinases (PI3Ks) phosphorylate inositol lipids and are involved in the immune response. The protein encoded by this gene is a class I PI3K found primarily in leukocytes. Like other class I PI3Ks (p110-alpha p110-beta, and p110-gamma), the encoded protein binds p85 adapter proteins and GTP-bound RAS. However, unlike the other class I PI3Ks, this protein phosphorylates itself, not p85 protein.[provided by RefSeq, Jul 2010]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

Bioinformatics

• Gene Symbol: PIK3CD