PI 3-Kinase p110 beta/PIK3CB Antibody

Western Blot: PI 3-Kinase p110 beta/PIK3CB Antibody [NBP1-33116] - A. 30 ug PC-12 whole cell lysate/extract 5 % SDS-PAGE PI3K p110 beta antibody [N3C2], Internal dilution: 1:1000

Immunocytochemistry/Immunofluorescence: PI 3-Kinase p110 beta/PIK3CB Antibody [NBP1-33116] - Analysis of methanol-fixed HeLa, using antibody at 1:200 dilution.

Immunohistochemistry-Paraffin: PI 3-Kinase p110 beta/PIK3CB Antibody [NBP1-33116] - Paraffin-embedded rat testis. PI3K p110 beta antibody [N3C2], Internal diluted at 1:400.

Western Blot: PI 3-Kinase p110 beta/PIK3CB Antibody [NBP1-33116] - Sample (50 ug of whole cell lysate) A: C2C12 B: C2C12+AEA10mM antibody diluted at 1:1000.

Western Blot: PI 3-Kinase p110 beta/PIK3CB Antibody [NBP1-33116] - Sample (30 ug of whole cell lysate) A: H1299 7.5% SDS PAGE; antibody diluted at 1:1000.

Product Discontinued

PI 3-Kinase p110 beta/PIK3CB Antibody Summary

• Immunogen: Recombinant protein encompassing a sequence within the center region of human PI3 kinase p110 beta. The exact sequence is proprietary.
• Predicted Species: Chicken (91%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: PIK3CB
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Western Blot 1:500-1:3000
• Theoretical MW: 123 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Use in Bovine reported in scientific literature (PMID:33625929).

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: 0.1M Tris (pH 7), 0.1M Glycine, 10% Glycerol
• Preservative: 0.01% Thimerosal
• Purity: Immunogen affinity purified

Alternate Names for PI 3-Kinase p110 beta/PIK3CB Antibody

• P110BETA
• PI 3Kinase p110 beta
• PI 3-Kinase p110 beta
• PI3K
• PI3KBETA
• PIK3C1
• PIK3CB phosphatidylinositol-4,5-bisphosphate 3-kinase, catalytic subunit beta
• PIK3CB

Background

PI3-Kinases (PI3-Ks) are a family of lipid kinases that are implicated in signal transduction. PI3-K consists of two subunits; p85 and p10. The p85 subunit localize PI3-K activity to the plasma membrane while the p110 subunit contains the catalytic domain of PI3-K (1-2). Four isoforms of p110 has been found;

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Publications for PI 3-Kinase p110 beta/PIK3CB Antibody (NBP1-33116)(2)

Publications using NBP1-33116

• Chaudhuri P, Smith AH, Putta P et al. P110 alpha and P110 delta catalytic subunits of PI3 kinase regulate lysophosphatidylcholine-induced TRPC6 externalization American journal of physiology. Cell physiology 2021-02-24 [PMID: 33625929] (WB, Human, Bovine)
• Sinanoglu O, Yener AN, Ekici S et al. The protective effects of spirulina in cyclophosphamide induced nephrotoxicity and urotoxicity in rats. Urology 2012-12-01 [PMID: 22951000] (WB, Mouse)

Bioinformatics

• Gene Symbol: PIK3CB