PI 3-Kinase C2 beta Antibody

Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line and U2OS human osteosarcoma cell line. PVDF membrane was probed with 2 µg/mL of Goat Anti-Human PI 3-Kinase C2 beta Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7249) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for PI 3-Kinase C2 beta at approximately 185 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

PI 3-Kinase C2 beta was detected in immersion fixed HEK293 human embryonic kidney cell line using Goat Anti-Human PI 3-Kinase C2 beta Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7249) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Predator cell PIK3C2B colocalizes with PI(3)P during engulfment and is required for complete internalization.(A) CRISPR-Cas9 single-cell clone PIK3C2B knockouts of MCF-7 were screened by immunoblot. Clones indicated by asterisk were chosen for further testing. (B) Predator cell engulfment rates (left) and confluency as a measure of viability (right) for senescent MCF-7 parental cells and 3 PIK3C2B knockout clones were determined by time course imaging in a DOXO-NT culture. Underlying data can be found at S1 Data. (C) Time course live-cell imaging of a senescent MCF-7 cell expressing 2xFYVE-mCherry and PIK3C2B-GFP throughout the entire process of engulfing an NIR-MCF-7 cell, scale bar = 100 μm. (D) Left: Merged (top) and separate (middle, lower) color channels of axial planes from bottom to top of a senescent MCF-7 cell expressing 2xFYVE-mCherry and PIK3C2B-GFP, engulfing NIR-MCF-7 cells. Right, volume view reconstruction of the same image, scale bar = 10 μm. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/36279312), licensed under a CC-BY license. Not internally tested by R&D Systems.

• Reactivity: Hu
• Applications: WB, ICC/IF
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: LYOPH

PI 3-Kinase C2 beta Antibody Summary

• Immunogen: E. coli-derived recombinant human PI 3‑Kinase C2 beta
  Ser2-Ser134
  Accession # O00750
• Specificity: Detects human PI 3‑Kinase C2 beta in direct ELISAs and Western blots.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: PIK3C2B
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry 5-15 ug/mL
  • Western Blot 2 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Sterile PBS to a final concentration of 0.2 mg/mL.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for PI 3-Kinase C2 beta Antibody

• C2-PI3K
• C2-PI3KDKFZp686G16234
• EC 2.7.1
• EC 2.7.1.154
• phosphatidylinositol 3-kinase C2 domain-containing beta polypeptide
• phosphatidylinositol-4-phosphate 3-kinase C2 domain-containing subunit beta
• Phosphoinositide 3-kinase-C2-beta
• phosphoinositide-3-kinase, class 2, beta polypeptide
• PI 3Kinase C2 beta
• PI 3-Kinase C2 beta
• PI3K-C2beta
• PI3K-C2-beta
• PIK3C2B
• PTDINS-3-kinase C2 beta
• PtdIns-3-kinase C2 subunit beta

Background

PIK3C2 beta (Phosphadidylinositol-4-phosphate 3 kinase C2 domain-containing beta subunit; also HsC2-PI3K) is a 175-185 kDa member of the Class II PI3/PI4 kinase family of enzymes. It is widely expressed, being found in neurons, urinary transitional and columnar epithelium, and fibroblasts. PIK3C2 beta likely participates in growth factor, integrin and chemokine signaling by interacting with select cell membrane receptors, and appears to phosphorylate both phosphatidylinositol (PI) and PI 4‑monophosphate upon activation. Human PIK3C2 beta is 1634 amino acids (aa) in length. It contains an N-terminal Pro-rich region (aa 156-174), two PI3K Class II domains (aa 622-794 and 1505-1627), a PI3K catalytic region (aa 987-1340), one PX domain (aa 1365-1481) and an NLS (aa 1555-1565). There are two potential C2 beta isoform variants. One shows a deletion of aa 850-877, while another contains a three aa insertion after Gln1016. Over aa 2-134, human PIK3C2 beta shares 91% aa identity with mouse PIK3C2 beta .

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for PI 3-Kinase C2 beta Antibody (AF7249)(1)

Publication using AF7249

• WD Frey, AY Anderson, H Lee, JB Nguyen, EL Cowles, H Lu, JG Jackson Phosphoinositide species and filamentous actin formation mediate engulfment by senescent tumor cells PloS Biology, 2022-10-24;20(10):e3001858. 2022-10-24 [PMID: 36279312] (Western Blot, Human)

Bioinformatics

• Gene Symbol: PIK3C2B
• Uniprot:
  • O00750(Human)