PI 3-Kinase C2 beta Antibody (OTI3B1) [CoraFluor™ 1]

• Reactivity: Hu, Mu, Rt
• Applications: WB, IHC
• Clone: OTI3B1
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: CoraFluor 1

PI 3-Kinase C2 beta Antibody (OTI3B1) [CoraFluor™ 1] Summary

• Description: CoraFluor(TM) 1 is a high performance terbium-based TR-FRET (Time-Resolved Fluorescence Resonance Energy Transfer) or TRF (Time-Resolved Fluorescence) donor for high throughput assay development. CoraFluor(IM) 1 absorbs UV light at approximately 340 nm, and emits at approximately 490 nm, 545 nm, 585 nm and 620 nm. It is compatible with common acceptor dyes that absorb at the emission wavelengths of CoraFluor(TM) 1. CoraFluor(TM) 1 can be used for the development of robust and scalable TR-FRET binding assays such as target engagement, ternary complex, protein-protein interaction and protein quantification assays.
• Immunogen: Full length human recombinant protein of human PIK3C2B(NP_002637) produced in HEK293T cell.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: PIK3C2B
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Western Blot
• Application Notes: Optimal dilution of this antibody should be experimentally determined.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Packaging, Storage & Formulations

• Storage: Store at 4C in the dark. Do not freeze.
• Buffer: PBS
• Preservative: No Preservative
• Purity: Immunogen affinity purified

Notes

CoraFluor (TM) is a trademark of Bio-Techne Corp. Sold for research purposes only under agreement from Massachusetts General Hospital. US patent 2022/0025254

Alternate Names for PI 3-Kinase C2 beta Antibody (OTI3B1) [CoraFluor™ 1]

• C2-PI3K
• C2-PI3KDKFZp686G16234
• EC 2.7.1
• EC 2.7.1.154
• phosphatidylinositol 3-kinase C2 domain-containing beta polypeptide
• phosphatidylinositol-4-phosphate 3-kinase C2 domain-containing subunit beta
• Phosphoinositide 3-kinase-C2-beta
• phosphoinositide-3-kinase, class 2, beta polypeptide
• PI 3Kinase C2 beta
• PI 3-Kinase C2 beta
• PI3K-C2beta
• PI3K-C2-beta
• PIK3C2B
• PTDINS-3-kinase C2 beta
• PtdIns-3-kinase C2 subunit beta

Background

PIK3C2B is encoded by this gene belongs to the phosphoinositide 3-kinase (PI3K) family. PI3-kinases play roles in signaling pathways involved in cell proliferation, oncogenic transformation, cell survival, cell migration, and intracellular protein trafficking. This protein contains a lipid kinase catalytic domain as well as a C-terminal C2 domain, a characteristic of class II PI3-kinases. C2 domains act as calcium-dependent phospholipid binding motifs that mediate translocation of proteins to membranes, and may also mediate protein-protein interactions. The PI3-kinase activity of this protein is sensitive to low nanomolar levels of the inhibitor wortmanin. The C2 domain of this protein was shown to bind phospholipids but not Ca2+, which suggests that this enzyme may function in a calcium-independent manner.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: PIK3C2B