PI 3-Kinase C2 beta Antibody (3E5)

Western Blot: PI 3-Kinase C2 beta Antibody (3E5) [H00005287-M02] - PIK3C2B monoclonal antibody (M02), clone 3E5 Analysis of PIK3C2B expression in A-431.

Immunocytochemistry/Immunofluorescence: PI 3-Kinase C2 beta Antibody (3E5) [H00005287-M02] - Analysis of monoclonal antibody to PIK3C2B on A-431 cell. Antibody concentration 10 ug/ml.

ELISA: PI 3-Kinase C2 beta Antibody (3E5) [H00005287-M02] - PI 3-Kinase C2 beta Antibody (3E5) [H00005287-M02] - Detection limit for recombinant GST tagged PIK3C2B is approximately 1ng/ml as a capture antibody.

Product Discontinued

PI 3-Kinase C2 beta Antibody (3E5) Summary

• Description: Quality control test: Antibody Reactive Against Recombinant Protein.
• Immunogen: PIK3C2B (NP_002637, 1 a.a. ~ 110 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MSSTQDNGEHWKSLESVGISRKELAMAEALQMEYDALSRLRHDKEENRAKQNADPSLISWDEPGVDFYSKPAGRRTDLKLLRGLSGSDPTLNYNSLSPQEGPPNHSTSQG
• Specificity: PIK3C2B - phosphoinositide-3-kinase, class 2, beta polypeptide (3E5)
• Isotype: IgG2a Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: PIK3C2B
• Purity: Ascites

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunocytochemistry/ Immunofluorescence
  • Western Blot 1:500
• Application Notes: Antibody reactivity against cell lysate and recombinant protein for WB. It has also been used for IF and ELISA.

Reactivity Notes

Mouse reactivity reported in scientific literature (PMID: 22645315).

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: In 1x PBS, pH 7.4
• Preservative: No Preservative
• Purity: Ascites

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for PI 3-Kinase C2 beta Antibody (3E5)

• C2-PI3K
• C2-PI3KDKFZp686G16234
• EC 2.7.1
• EC 2.7.1.154
• phosphatidylinositol 3-kinase C2 domain-containing beta polypeptide
• phosphatidylinositol-4-phosphate 3-kinase C2 domain-containing subunit beta
• Phosphoinositide 3-kinase-C2-beta
• phosphoinositide-3-kinase, class 2, beta polypeptide
• PI 3Kinase C2 beta
• PI 3-Kinase C2 beta
• PI3K-C2beta
• PI3K-C2-beta
• PIK3C2B
• PTDINS-3-kinase C2 beta
• PtdIns-3-kinase C2 subunit beta

Background

The protein encoded by this gene belongs to the phosphoinositide 3-kinase (PI3K) family. PI3-kinases play roles in signaling pathways involved in cell proliferation, oncogenic transformation, cell survival, cell migration, and intracellular protein trafficking. This protein contains a lipid kinase catalytic domain as well as a C-terminal C2 domain, a characteristic of class II PI3-kinases. C2 domains act as calcium-dependent phospholipid binding motifs that mediate translocation of proteins to membranes, and may also mediate protein-protein interactions. The PI3-kinase activity of this protein is sensitive to low nanomolar levels of the inhibitor wortmanin. The C2 domain of this protein was shown to bind phospholipids but not Ca2+, which suggests that this enzyme may function in a calcium-independent manner.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for PI 3-Kinase C2 beta Antibody (H00005287-M02)(1)

Publication using H00005287-M02

• Srivastava S, Cai X, Li Z, Sun Y, Skolnik EY. Phosphatidylinositol-3-Kinase C2 beta and TRIM27 Function To Positively and Negatively Regulate IgE Receptor Activation of Mast Cells Mol Cell Biol 2012-08-01 [PMID: 22645315] (WB, Mouse)

Bioinformatics

• Gene Symbol: PIK3C2B
• OMIM:
  • 602838(Human)