Phosphorylase B Antibody - BSA Free

Immunohistochemistry: Phosphorylase B Antibody - BSA Free [NBP3-47160] - Immunohistochemistry of paraffin-embedded human skeletal muscle tissue slide using (Phosphorylase B Antibody) at dilution of 1:50

Immunoprecipitation: Phosphorylase B Antibody - BSA Free [NBP3-47160] - IP Result of anti-Phosphorylase B (IP:, 4ug; Detection: 1:500) with mouse heart tissue lysate 3200ug.

Western Blot: Phosphorylase B Antibody - BSA Free [NBP3-47160] - Mouse heart tissue were subjected to SDS PAGE followed by western blot with (Phosphorylase B antibody) at dilution of 1:1000

• Reactivity: Hu, Mu
• Applications: WB, ELISA, IHC, IP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

Phosphorylase B Antibody - BSA Free Summary

• Immunogen: phosphorylase kinase, beta. (Uniprot# Q93100)
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: PHKB
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunohistochemistry 1:20-1:200
  • Immunoprecipitation 1:200-1:1000
  • Western Blot 1:500-1:2000
• Theoretical MW: 124 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS, 50% Glycerol, pH (7.3)
• Preservative: 0.02% Sodium Azide
• Purity: Immunogen affinity purified

Alternate Names for Phosphorylase B Antibody - BSA Free

• DKFZp781E15103
• FLJ41698
• phosphorylase b kinase regulatory subunit beta
• phosphorylase kinase beta-subunit
• Phosphorylase kinase subunit beta
• phosphorylase kinase, beta

Background

Phosphorylase B, also known by its gene name, PHKB, is an enzyme localized to the plasma membrane that catalyzes the phosphorylation of the amino acid residue Serine. Phosphorylase B has a 1,093 amino acid long isoform that is 125 kDa (the canonical sequence) and three other isoforms derived from alternative splicing. Current research on PHKB is being performed relating to several diseases and disorders including hepatitis and metabolic disorders, such as hypoglycemia and glycogen storage disease. Phosphorylase B has also been shown to have interactions with B Raf, hnRNP C1 + C2, PASK, and UBE3A in pathways such as Glucose and Glycogen Metabolism, PKA Signaling, cAMP Pathway, Insulin Signaling Pathway and Calcium Signaling Pathway.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: PHKB