PHKA1 Antibody - BSA Free

Western Blot: PHKA1 Antibody [NBP1-87281] - Lane 1: Marker [kDa] 219, 112, 85, 49, 32, 25, 18. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG sp. Lane 4: Human cell line A-431. Lane 5: Human liver tissue. Lane 6: Human tonsil tissue

Immunocytochemistry/Immunofluorescence: PHKA1 Antibody [NBP1-87281] - Immunofluorescent staining of human cell line A-431 shows localization to vesicles.

Immunohistochemistry-Paraffin: PHKA1 Antibody [NBP1-87281] - Staining of human small intestine shows low expression as expected.

Immunohistochemistry-Paraffin: PHKA1 Antibody [NBP1-87281] - Staining of human pancreas shows strong granular cytoplasmic positivity in exocrine glandular cells.

Immunohistochemistry-Paraffin: PHKA1 Antibody [NBP1-87281] - Staining of human parathyroid gland shows high expression.

Immunohistochemistry-Paraffin: PHKA1 Antibody [NBP1-87281] - Staining in human parathyroid gland and small intestine tissues using anti-PHKA1 antibody. Corresponding PHKA1 RNA-seq data are presented for the same tissues.

• Reactivity: Hu, Mu
• Applications: WB, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

PHKA1 Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit PHKA1 Antibody - BSA Free (NBP1-87281) is a polyclonal antibody validated for use in IHC, WB and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: This antibody was developed against Recombinant Protein corresponding to amino acids: AHSLRCSAEEATEGLMNLSPSAMKNLLHHILSGKEFGVERSVRPTDSNVSPAISIHEIGAVGATKTERTGIMQLKSEIKQSPGTSMTPSSGSFPSAYDQQSSKDSRQGQWQ
• Predicted Species: Mouse (90%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: PHKA1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 0.25-2 ug/ml
  • Immunohistochemistry 1:50 - 1:200
  • Immunohistochemistry-Paraffin 1:50 - 1:200
  • Western Blot 0.04-0.4 ug/ml
• Application Notes: For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.
• Control Peptide:

  PHKA1 Protein (NBP1-87281PEP)

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Rat (80%)

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.2) and 40% Glycerol
• Preservative: 0.02% Sodium Azide
• Purity: Immunogen affinity purified

Alternate Names for PHKA1 Antibody - BSA Free

• MGC132604
• PHKA
• phosphorylase b kinase regulatory subunit alpha skeletal muscle isoform
• phosphorylase b kinase regulatory subunit alpha, skeletal muscle isoform
• Phosphorylase kinase alpha M subunit
• phosphorylase kinase, alpha 1 (muscle)
• phosphorylase kinase, alpha 1 (muscle), muscle glycogenosis

Background

The PHKA1 gene encodes the alpha subunit of muscle phosphorylase kinase (EC 2.7.1.38), a key regulatory enzyme of glycogen metabolism. Phosphorylase kinase consists of 4 copies of an alpha-beta-gamma-delta tetramer. The alpha, beta (PHKB; MIM 172490), and gamma (PHKG1; MIM 172470 and PHKG2; MIM 172471) subunits have several isoforms; the delta subunit is calmodulin (CALM1; MIM 114180). PHKA2 (MIM 306000) encodes the alpha subunit of liver-specific phosphorylase kinase and is also located on the X chromosome.[supplied by OMIM]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: PHKA1