Phosphodiesterase 4A/PDE4A [p Ser686, p Ser688] Antibody

Western Blot: Phosphodiesterase 4A/PDE4A [p Ser686, p Ser688] Antibody [NB300-635] - Lane 1: PDE4A Western blot positive Control (PDE4A5); Lane 2: Western blot with Phospho-PDE4A5

• Reactivity: Hu, Mu, Rt, Rb
• Applications: WB, ELISA, IP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated

Phosphodiesterase 4A/PDE4A [p Ser686, p Ser688] Antibody Summary

• Immunogen: Synthetic peptide corresponding to PKA phosphorylation site on PDE4A long form enzymes. The peptide was synthesized with phosphorylated serine.
• Modification: p Ser686, p Ser688
• Localization: Cell Membrane
• Specificity: This detects all known long-from variants of PDE4A, including PDE4A4, PDE4A5, PDE4A8, PDE4Ax when phosphorylated at the PKA phophorylation site. It will not cross-react with PDE4A phosphorylated at the ERK phosphorylation site. It does not cross-react with PDE4B, PDE4D or any other member of the PDE family.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: PDE4A
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:10000
  • Immunoprecipitation 1:150
  • Western Blot 1:500
• Application Notes: Immunoprecipitation and Western Blot: Detects bands at 66 kDa (PDE4A1), 109 kDa (PDE4A5), 106 kDa(PDE4A8), 102 kDa (PDE4Ax) and 76 kDa (testis specific PDE4A variant). Optimal dilutions and concentrations should be determined by the end user.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: Tris/Glycine buffer pH 7.5-7.9, stabilizing protein, and glycerol
• Preservative: 0.02% Sodium Azide
• Purity: Immunogen affinity purified

Alternate Names for Phosphodiesterase 4A/PDE4A [p Ser686, p Ser688] Antibody

• cAMP-specific 3'-5'-cyclic phosphodiesterase 4A
• DPDE2
• DPDE2cAMP-specific phosphodiesterase
• EC 3.1.4
• EC 3.1.4.17
• PDE4
• PDE46
• PDE4A
• Phosphodiesterase 4A
• phosphodiesterase 4A, cAMP-specific (dunce (Drosophila)-homologphosphodiesterase E2)
• phosphodiesterase 4A, cAMP-specific (dunce
• phosphodiesterase 4A, cAMP-specific
• phosphodiesterase E2 dunce homolog, Drosophila
• phosphodiesterase isozyme 4

Background

Enzymes of the cAMP-dependent phosphodiesterase type 4 (PDE4) family are important in hydrolyzing cAMP produced by G-protein coupled receptor (GPCR) stimulated adenylyl cyclases. In brain, more than 90% of cAMP formed by the stimulation of GPCRs is hydrolyzed by PDE4 enzymes. PDE4 enzymes are also important molecular targets for a variety of therapeutic agents like antidepressants, anti-asthmatics, and anti-inflammatory drugs. PDE4 family comprises 4 genes (PDE4A, B, C and D); each exhibiting multiple isozymes due to alternate splicing that leads to a larger number of distinct PDE4 variants. Members of the PDE4 family are regulated/activated by phosphorylation/dephosphorylation by cAMP-dependent protein kinase A and phosphatases. Protein-protein interactions and cellular trafficking of PDE4A enzymes play an important role in cAMP compartmentalization and cAMP-dependent signaling. In brain members of the PDE4A, B and D family are associated with GPCRs (adrenergic and dopaminergic) signaling.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Phosphodiesterase 4A/PDE4A Antibody (NB300-635)(1)

Publication using NB300-635

• Ale-Agha N, Goy C, Jakobs P et al. CDKN1B/p27 is localized in mitochondria and improves respiration-dependent processes in the cardiovascular system-New mode of action for caffeine PLoS Biol. Jun 1 2018 [PMID: 29927970] (WB, Mouse)

Bioinformatics

• Gene Symbol: PDE4A
• Entrez:
  • 5141(Human)
  • 18577(Mouse)
  • 25638(Rat)
• Uniprot:
  • P27815(Human)