Phosphodiesterase 4A/PDE4A [p Ser686, p Ser688] Antibody

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Western Blot: Phosphodiesterase 4A/PDE4A [p Ser686, p Ser688] Antibody [NB300-635] - Lane 1: PDE4A Western blot positive Control (PDE4A5); Lane 2: Western blot with Phospho-PDE4A5

Product Details

Summary
Reactivity Hu, Mu, Rt, RbSpecies Glossary
Applications WB, ELISA, IP
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

Phosphodiesterase 4A/PDE4A [p Ser686, p Ser688] Antibody Summary

Immunogen
Synthetic peptide corresponding to PKA phosphorylation site on PDE4A long form enzymes. The peptide was synthesized with phosphorylated serine.
Modification
p Ser686, p Ser688
Localization
Cell Membrane
Specificity
This detects all known long-from variants of PDE4A, including PDE4A4, PDE4A5, PDE4A8, PDE4Ax when phosphorylated at the PKA phophorylation site. It will not cross-react with PDE4A phosphorylated at the ERK phosphorylation site. It does not cross-react with PDE4B, PDE4D or any other member of the PDE family.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
PDE4A
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • ELISA 1:10000
  • Immunoprecipitation 1:150
  • Western Blot 1:500
Application Notes
Immunoprecipitation and Western Blot: Detects bands at 66 kDa (PDE4A1), 109 kDa (PDE4A5), 106 kDa(PDE4A8), 102 kDa (PDE4Ax) and 76 kDa (testis specific PDE4A variant). Optimal dilutions and concentrations should be determined by the end user.
Publications
Read Publication using
NB300-635 in the following applications:

  • WB
    1 publication

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
Tris/Glycine buffer pH 7.5-7.9, stabilizing protein, and glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for Phosphodiesterase 4A/PDE4A [p Ser686, p Ser688] Antibody

  • cAMP-specific 3'-5'-cyclic phosphodiesterase 4A
  • DPDE2
  • DPDE2cAMP-specific phosphodiesterase
  • EC 3.1.4
  • EC 3.1.4.17
  • PDE4
  • PDE46
  • PDE4A
  • Phosphodiesterase 4A
  • phosphodiesterase 4A, cAMP-specific (dunce (Drosophila)-homologphosphodiesterase E2)
  • phosphodiesterase 4A, cAMP-specific (dunce
  • phosphodiesterase 4A, cAMP-specific
  • phosphodiesterase E2 dunce homolog, Drosophila
  • phosphodiesterase isozyme 4

Background

Enzymes of the cAMP-dependent phosphodiesterase type 4 (PDE4) family are important in hydrolyzing cAMP produced by G-protein coupled receptor (GPCR) stimulated adenylyl cyclases. In brain, more than 90% of cAMP formed by the stimulation of GPCRs is hydrolyzed by PDE4 enzymes. PDE4 enzymes are also important molecular targets for a variety of therapeutic agents like antidepressants, anti-asthmatics, and anti-inflammatory drugs. PDE4 family comprises 4 genes (PDE4A, B, C and D); each exhibiting multiple isozymes due to alternate splicing that leads to a larger number of distinct PDE4 variants. Members of the PDE4 family are regulated/activated by phosphorylation/dephosphorylation by cAMP-dependent protein kinase A and phosphatases. Protein-protein interactions and cellular trafficking of PDE4A enzymes play an important role in cAMP compartmentalization and cAMP-dependent signaling. In brain members of the PDE4A, B and D family are associated with GPCRs (adrenergic and dopaminergic) signaling.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Phosphodiesterase 4A/PDE4A Antibody (NB300-635)(1)

We have publications tested in 1 confirmed species: Mouse.

We have publications tested in 1 application: WB.


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Bioinformatics

Gene Symbol PDE4A
Entrez
Uniprot