Phosphodiesterase 4D Antibody

Western Blot: Phosphodiesterase 4D Antibody [NB100-2462] - HEK293 overexpressing Human PDE4D2 and probed with (mock transfection in first lane).

Immunohistochemistry-Paraffin: Phosphodiesterase 4D Antibody [NB100-2462] - staining of paraffin embedded Human Skeletal Muscle. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Western Blot: Phosphodiesterase 4D Antibody [NB100-2462] - (1ug/ml) of COS cell lysates (25ug protein): transfected with Human PDE4D1 (1), transfected with Human PDE4D3 (2), untransfected (3). Primary incubation was 1 hour. Detected by chemiluminescence.

Immunohistochemistry-Paraffin: Phosphodiesterase 4D Antibody [NB100-2462] - staining of paraffin embedded Human Heart. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

• Reactivity: Hu, Mu
• Applications: WB, ELISA, IHC, IHC-P, PEP-ELISA
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

Phosphodiesterase 4D Antibody Summary

• Immunogen: Peptide with sequence QPEACVIDDRSPDT corresponding to C-Terminus according to NP_001184151.1, NP_001184150.1, NP_006194.2, NP_001098101.1, NP_001184152.1, NP_001159371.1.
• Specificity: This antiobody is expected to recognize all reported isoforms.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: PDE4D
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:200
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 5 ug/ml
  • Peptide ELISA Detection limit 1:128000
  • Western Blot 0.1 - 0.3 ug/ml
• Application Notes: ELISA successful usage is reported in scientific literature (PMID: 19939455). WB: Approx. 70+80+90 kDa band bands observed in lysates of A549. In transfected COS1 transiently expressing PDE4D1 and PDE4D3 bands of approx. 68 kDa and 95 kDa are observed. These bands are not observed in the untransfected COS1. In transfected HEK293 transiently expressing PDE4D2 multi bands from approx. 55 kDa to 80 kDa are observed. These bands are not observed in the non-transfected HEK293. Calculated MW of 91.1 kDa band according to PDE4D1 (NP_001098101.1), 76.5 kDa band according to PDE4D2 (NP_006194.2) and 84.7 kDa band according to PDE4D3 (NP_001159371.1).
  IHC: Paraffin embedded Human Skeletal Muscle and Heart.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for Phosphodiesterase 4D Antibody

• cAMP-specific (dunce (Drosophila)-homologphosphodiesterase E3)
• cAMP-specific 3'-5'-cyclic phosphodiesterase 4D
• cAMP-specific phosphodiesterase PDE4D6
• DKFZp686M11213
• DPDE3
• Drosophila)
• dunce-like phosphodiesterase E3
• EC 3.1.4
• FLJ97311
• HSPDE4D
• PDE43
• PDE4D
• Phosphodiesterase 4D
• phosphodiesterase 4D, cAMP-specific (phosphodiesterase E3 dunce homolog
• phosphodiesterase 4D, cAMP-specific

Background

Gene ID(s): 5144 (human)

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Phosphodiesterase 4D Antibody (NB100-2462)(2)

Publications using NB100-2462

• Bazhin AV, Kahnert S, Kimpfler S et al. Distinct metabolism of cyclic adenosine monophosphate in regulatory and helper CD4(+) T cells. Mol Immunol 2009-11-23 [PMID: 19939455]
• Houslay MD. The long and short of vascular smooth muscle phosphodiesterase-4 as a putative therapeutic target. Mol Pharmacol 2005-09-01 [PMID: 15958394]

Bioinformatics

• Gene Symbol: PDE4D
• Entrez:
  • 5144(Human)
• Uniprot:
  • NP_006194.2(Human)