Phosphodiesterase 4D Antibody

Images

 
Western Blot: Phosphodiesterase 4D Antibody [NB100-2462] - HEK293 overexpressing Human PDE4D2 and probed with (mock transfection in first lane).
Immunohistochemistry-Paraffin: Phosphodiesterase 4D Antibody [NB100-2462] - staining of paraffin embedded Human Skeletal Muscle. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Western Blot: Phosphodiesterase 4D Antibody [NB100-2462] - (1ug/ml) of COS cell lysates (25ug protein): transfected with Human PDE4D1 (1), transfected with Human PDE4D3 (2), untransfected (3). Primary incubation was 1 ...read more
Immunohistochemistry-Paraffin: Phosphodiesterase 4D Antibody [NB100-2462] - staining of paraffin embedded Human Heart. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, ELISA, IHC, IHC-P, PEP-ELISA
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
0.5 mg/ml

Order Details

Phosphodiesterase 4D Antibody Summary

Immunogen
Peptide with sequence QPEACVIDDRSPDT corresponding to C-Terminus according to NP_001184151.1, NP_001184150.1, NP_006194.2, NP_001098101.1, NP_001184152.1, NP_001159371.1.
Specificity
This antiobody is expected to recognize all reported isoforms.
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
PDE4D
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 0.1 - 0.3 ug/ml
  • ELISA 1:200
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 5 ug/ml
  • Peptide ELISA Detection limit 1:128000
Application Notes
ELISA successful usage is reported in scientific literature (PMID: 19939455). WB: Approx. 70+80+90 kDa band bands observed in lysates of A549. In transfected COS1 transiently expressing PDE4D1 and PDE4D3 bands of approx. 68 kDa and 95 kDa are observed. These bands are not observed in the untransfected COS1. In transfected HEK293 transiently expressing PDE4D2 multi bands from approx. 55 kDa to 80 kDa are observed. These bands are not observed in the non-transfected HEK293. Calculated MW of 91.1 kDa band according to PDE4D1 (NP_001098101.1), 76.5 kDa band according to PDE4D2 (NP_006194.2) and 84.7 kDa band according to PDE4D3 (NP_001159371.1).
IHC: Paraffin embedded Human Skeletal Muscle and Heart.
Publications
Read Publications using NB100-2462.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Purity
Immunogen affinity purified

Alternate Names for Phosphodiesterase 4D Antibody

  • cAMP-specific (dunce (Drosophila)-homologphosphodiesterase E3)
  • cAMP-specific 3'-5'-cyclic phosphodiesterase 4D
  • cAMP-specific phosphodiesterase PDE4D6
  • DKFZp686M11213
  • Drosophila)
  • dunce-like phosphodiesterase E3
  • EC 3.1.4
  • FLJ97311
  • HSPDE4D
  • phosphodiesterase 4D, cAMP-specific (phosphodiesterase E3 dunce homolog
  • phosphodiesterase 4D, cAMP-specific

Background

Gene ID(s): 5144 (human)

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Phosphodiesterase 4D Antibody (NB100-2462)(2)

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Bioinformatics

Gene Symbol PDE4D
Entrez
Uniprot