Phospho-p38 alpha (T180/Y182) DuoSet IC ELISA, 2 Plate

• Reactivity: Hu, Mu, Rt
• Applications: ELISA
• Conjugate: Biotin

Phospho-p38 alpha (T180/Y182) DuoSet IC ELISA, 2 Plate Summary

• Modification: p Thr180, p Tyr182
• Source: N/A
• Assay Type: Solid Phase Sandwich ELISA
• Inter-Assay: See PDF Datasheet for details
• Intra-Assay: See PDF Datasheet for details
• Spike Recovery: See PDF Datasheet for details
• Sample Volume: See PDF Datasheet for details
• Gene: MAPK14

Applications/Dilutions

• Dilutions:
  • ELISA
• Application Notes: No significant interference observed with available related molecules.

Packaging, Storage & Formulations

• Storage: Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Phospho-p38 alpha (T180/Y182) DuoSet IC ELISA, 2 Plate

• CSBP;CSBP1;CSBP2;CSPB1;EXIP;MAPK14;Mitogen-activated protein kinase;Mitogen-activated protein kinase 14;Mxi2;p38;p38ALPHA;PRKM14;PRKM15;RK;SAPK2A
• MAPK14
• p38 alpha
• SAPK2a

Background

The p38 Mitogen-activated Protein Kinases (MAPKs) are a family of four related Ser/Thr kinases activated by proinflammatory cytokines and environmental stresses. All four p38 family members, alpha, beta, gamma, and delta, are phosphorylated by MKK3 and/or MKK6 at dual Thr and Tyr positions within the phosphoacceptor sequence Thr-Gly-Tyr. Once activated, p38 phosphorylates a number of targets, including the nuclear transcription factors ATF2 and Max.

The most frequently analyzed family member, p38 alpha, also known as SAPK2a and MAPK14, was initially purified as a kinase critical to the signaling cascade linking IL-1 to MAPKAPK-2 and the small heat shock protein HSP27. Ubiquitously expressed, p38 alpha is dually phosphorylated by MKK3 and MKK6 at Thr180 and Tyr182. Once activated, p38 alpha phosphorylates a number of targets, including the cytoplasmic kinases MNK 4 and PRAK5 and the nuclear transcription factors ATF2 1 and STAT1. Several promising compounds that inhibit p38 alpha are being investigated as potential therapies for arthritic and inflammatory diseases.

Publications for p38 alpha (DYC869B-2)(15)

Publications using DYC869B-2

• Zhong, S;Wang, R;Gao, X;Guo, Q;Lin, R;Luo, M; Modular DNA barcoding of nanobodies enables multiplexed in situ protein imaging and high-throughput biomolecule detection eLife 2025-07-22 [PMID: 40694500] (Human)
• G Feigelman, E Simanovich, P Brockmeyer, MA Rahat Knocking-Down CD147/EMMPRIN Expression in CT26 Colon Carcinoma Forces the Cells into Cellular and Angiogenic Dormancy That Can Be Reversed by Interactions with Macrophages Biomedicines, 2023-03-02;11(3):. 2023-03-02 [PMID: 36979746] (Mouse)
• J Knobloch, D Jungck, J Kronsbein, E Stoelben, K Ito, A Koch LABAs and p38MAPK Inhibitors Reverse the Corticosteroid-Insensitivity of IL-8 in Airway Smooth Muscle Cells of COPD J Clin Med, 2019-11-22;8(12):. 2019-11-22 [PMID: 31766770] (Human)
• Q Ma, Q Liu, L Yuan, Y Zhuang Protective Effects of LSGYGP from Fish Skin Gelatin Hydrolysates on UVB-Induced MEFs by Regulation of Oxidative Stress and Matrix Metalloproteinase Activity Nutrients, 2018-03-28;10(4):. 2018-03-28 [PMID: 29597313] (Mouse)
• Ghezali L, Leger D, Limami Y, Cook-Moreau J, Beneytout J, Liagre B Cyclopamine and jervine induce COX-2 overexpression in human erythroleukemia cells but only cyclopamine has a pro-apoptotic effect. Exp Cell Res, 2013-01-25;319(7):1043-53. 2013-01-25 [PMID: 23357584] (Human)
• Holenya P, Kitanovic I, Heigwer F, Wolfl S Microarray-based kinetic colorimetric detection for quantitative multiplex protein phosphorylation analysis. Proteomics, 2011-04-18;11(10):2129-33. 2011-04-18 [PMID: 21500346] (Human)
• Gouwy M, Struyf S, Berghmans N, Vanormelingen C, Schols D, Van Damme J CXCR4 and CCR5 ligands cooperate in monocyte and lymphocyte migration and in inhibition of dual-tropic (R5/X4) HIV-1 infection. Eur. J. Immunol., 2011-03-07;41(4):963-73. 2011-03-07 [PMID: 21381021] (Human)
• Yeh CC, Li H, Malhotra D, Turcato S, Nicholas S, Tu R, Zhu BQ, Cha J, Swigart PM, Myagmar BE, Baker AJ, Simpson PC, Mann MJ Distinctive ERK and p38 signaling in remote and infarcted myocardium during post-MI remodeling in the mouse. J. Cell. Biochem., 2010-04-15;109(6):1185-91. 2010-04-15 [PMID: 20186881] (Mouse)
• Montalban AG, Boman E, Chang CD, Ceide SC, Dahl R, Dalesandro D, Delaet NG, Erb E, Ernst J, Gibbs A, Kahl J, Kessler L, Lundstrom J, Miller S, Nakanishi H, Roberts E, Saiah E, Sullivan R, Wang Z, Larson CJ KR-003048, a potent, orally active inhibitor of p38 mitogen-activated protein kinase. Eur. J. Pharmacol., 2010-02-02;632(1):93-102. 2010-02-02 [PMID: 20132813] (Human)
• Hsieh CH, Nickel EA, Chen J, Schwacha MG, Choudhry MA, Bland KI, Chaudry IH Mechanism of the salutary effects of estrogen on kupffer cell phagocytic capacity following trauma-hemorrhage: pivotal role of Akt activation. J. Immunol., 2009-04-01;182(7):4406-14. 2009-04-01 [PMID: 19299741] (Mouse)
• Muselet-Charlier C, Roque T, Boncoeur E, Chadelat K, Clement A, Jacquot J, Tabary O Enhanced IL-1beta-induced IL-8 production in cystic fibrosis lung epithelial cells is dependent of both mitogen-activated protein kinases and NF-kappaB signaling. Biochem. Biophys. Res. Commun., 2007-04-02;357(2):402-7. 2007-04-02 [PMID: 17420005] (Human)
• Leger DY, Liagre B, Beneytout JL Low dose leflunomide activates PI3K/Akt signalling in erythroleukemia cells and reduces apoptosis induced by anticancer agents. Apoptosis, 2006-10-01;11(10):1747-60. 2006-10-01 [PMID: 16927021] (Human)
• Tacker DH, Herzog NK, Okorodudu AO Cocaethylene affects human microvascular endothelial cell p38 mitogen-activated protein kinase activation and nuclear factor-kappaB DNA-binding activity. Clin. Chem., 2006-08-17;52(10):1926-33. 2006-08-17 [PMID: 16916993] (Human)
• Vergne-Salle</LastName><F P</Initial, Vergne-Salle P, Leger DY, Bertin P, Treves R, Beneytout JL, Liagre B Effects of the active metabolite of leflunomide, A77 1726, on cytokine release and the MAPK signalling pathway in human rheumatoid arthritis synoviocytes. Cytokine, 2005-09-07;31(5):335-48. 2005-09-07 [PMID: 16099671] (Human)
• Studer RK, Bergman R, Stubbs T, Decker K Chondrocyte response to growth factors is modulated by p38 mitogen-activated protein kinase inhibition. Arthritis Res. Ther., 2003-11-07;6(1):R56-R64. 2003-11-07 [PMID: 14979938] (Rabbit)

FAQs for p38 alpha (DYC869B-2). (Showing 1 - 1 of 1 FAQs).

1. What is the difference between the Quantikine and Duoset ELISA kits?
   • Our Quantikine kits are fully optimized and validated for the sample types listed on the product specific webpage and datasheet, as this can vary between kits. Each kit is supplied ready to use with one pre-coated 96-well plate, a detection antibody directly conjugated to HRP, and all other necessary reagents. These kits are ideal for researchers who want the convenience of a ready to use and optimized ELISA product. Some of these kits are also available prepackaged in larger 6 and 50 plate sizes.Our DuoSet Kits, in contrast, are ELISA development kits containing the capture and detection antibody, the mass-value calibrated standard, and streptavidin-HRP to prepare approximately 5 or 15 plates. Ancillary reagents will need to be used/purchased, and for most kits, we will recommend one of our Ancillary Reagent Kits, which contain the reagents we use ourselves in-house. DuoSet kits are validated only for cell culture supernatant samples and therefore require further development and validation for accurate measurement in more complex samples such as serum and plasma. Our DuoSet Kits offer an economical, flexible alternative for the experienced ELISA user.

Bioinformatics

• Gene Symbol: MAPK14