PHLPP2 Antibody - BSA Free

Immunocytochemistry/Immunofluorescence: PHLPP2 Antibody [NBP2-13757] - Staining of human cell line HEK 293 shows localization to vesicles.

Immunohistochemistry-Paraffin: PHLPP2 Antibody [NBP2-13757] - Staining of human spleen shows strong positivity in a subset of cells in the red pulp.

• Reactivity: Hu, Mu
• Applications: ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

PHLPP2 Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit PHLPP2 Antibody - BSA Free (NBP2-13757) is a polyclonal antibody validated for use in IHC and ICC/IF. Anti-PHLPP2 Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: This antibody was developed against a recombinant protein corresponding to the amino acids: DLHLVLCTVETPASEICAGEGRESLYLQLHGDLVRRLEPTERPLQIVYDYLSRLGFDDPVRIQEEATNPDLGCMIRFYG
• Predicted Species: Mouse (96%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: PHLPP2
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 0.25-2 ug/ml
  • Immunohistochemistry 1:50 - 1:200
  • Immunohistochemistry-Paraffin 1:50 - 1:200
• Application Notes: For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF, Fixation Permeabilization: Use PFA/Triton X-100.
• Control Peptide:

  PHLPP2 Protein (NBP2-13757PEP)

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.2) and 40% Glycerol
• Preservative: 0.02% Sodium Azide
• Purity: Immunogen affinity purified

Alternate Names for PHLPP2 Antibody - BSA Free

• EC 3.1.3.16
• KIAA0931PH domain leucine-rich repeat-containing protein phosphatase 2
• PH domain and leucine rich repeat protein phosphatase 2
• PH domain and leucine rich repeat protein phosphatase-like
• PH domain leucine-rich repeat-containing protein phosphatase-like
• PHLPPLPHLPP-like

Background

PHLPP2 contains 21 LRR (leucine rich) repeats, 1 PH domain and 1 PP2C like domain. It is a protein phosphatase that specifically mediates dephosphorylation of AKT1 Ser 473, a protein that regulates the balance between cell survival and apoptosis through a cascade that primarily alters the function of transcription factors that regulate pro and antiapoptotic genes. Dephosphorylation of Ser 473 of AKT1 triggers apoptosis and decrease cell proliferation. PHLPP2 also controls the phosphorylation of AKT3. It binds 2 manganese ions per subunit. There are 3 named isoforms produced by alternative splicing.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for PHLPP2 Antibody (NBP2-13757)(1)

Publication using NBP2-13757

• Kang D, Hwang HJ, Baek Y et Al. TRIM22 induces cellular senescence by targeting PHLPP2 in hepatocellular carcinoma Cell Death Dis 2024-01-10 [PMID: 38199981]

Bioinformatics

• Gene Symbol: PHLPP2