PHKG2 Antibody (2B8) - Azide and BSA Free

Western Blot: PHKG2 Antibody (2B8) [H00005261-M05] - Analysis of PHKG2 expression in transfected 293T cell line by PHKG2 monoclonal antibody (M05), clone 2B8. Lane 1: PHKG2 transfected lysate (Predicted MW: 46.4 KDa). Lane 2: Non-transfected lysate.

Immunocytochemistry/Immunofluorescence: PHKG2 Antibody (2B8) [H00005261-M05] - Analysis of monoclonal antibody to PHKG2 on HeLa cell. Antibody concentration 10 ug/ml

Sandwich ELISA: PHKG2 Antibody (2B8) [H00005261-M05] - Detection limit for recombinant GST tagged PHKG2 is approximately 10ng/ml as a capture antibody.

• Reactivity: Hu
• Applications: WB, ELISA, ICC/IF
• Clone: 2B8
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free

PHKG2 Antibody (2B8) - Azide and BSA Free Summary

• Description: Quality control test: Antibody Reactive Against Recombinant Protein.
• Immunogen: PHKG2 (AAH02541.1, 1 a.a. ~ 406 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MTLDVGPEDELPDWAAAKEFYQKYDPKDVIGRGVSSVVRRCVHRATGHEFAVKIMEVTAERLSPEQLEEVREATRRETHILRQVAGHPHIITLIDSYESSSFMFLVFDLMRKGELFDYLTEKVALSEKETRSIMRSLLEAVSFLHANNIVHRDLKPENILLDDNMQIRLSDFGFSCHLEPGEKLRELCGTPGYLAPEILKCSMDETHPGYGKEVDLWACGVILFTLLAGSPPFWHRRQILMLRMIMEGQYQFSSPEWDDRSSTVKDLISRLLQVDPEARLTAEQALQHPFFERCEGSQPWNLTPRQRFRVAVWTVLAAGRVALSTHRVRPLTKNALLRDPYALRSVRHLIDNCAFRLYGHWVKKGEQQNRAALFQHRPPGPFPIMGPEEEGDSAAITEDEAVLVLG
• Specificity: PHKG2 (2B8)
• Isotype: IgG2a Lambda
• Clonality: Monoclonal
• Host: Mouse
• Gene: PHKG2
• Purity: IgG purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunocytochemistry/ Immunofluorescence
  • Sandwich ELISA
  • Western Blot 1:500
• Application Notes: Antibody reactivity against Recombinant Protein with GST tag on ELISA and WB. GST tag alone is used as a negative control.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: In 1x PBS, pH 7.4
• Preservative: No Preservative
• Purity: IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for PHKG2 Antibody (2B8) - Azide and BSA Free

• EC 2.7.11
• EC 2.7.11.19
• GSD9C
• PHK-gamma-T
• phosphorylase b kinase gamma catalytic chain, testis/liver isoform
• Phosphorylase kinase subunit gamma-2
• phosphorylase kinase, gamma 2 (testis)
• Phosphorylase kinase, gamma 2 (testis/liver)
• PSK-C3

Background

PHKG2, also known as Phosphorylase b kinase gamma catalytic chain, liver/testis isoform, has 2 isoforms, a 406 amino acid long isoform that is 46 kDa and a short 374 amino acid isoform that is 43 kDa, as catalytic subunit of the phosphorylase b kinase (PHK) mediates the neural and hormonal regulation of glycogen breakdown (glycogenolysis) by phosphorylating and thereby activating glycogen phosphorylase and may regulate glycogeneolysis in the testis and phosphorylates PYGM in vitro. Studies on this protein have shown a relationship with the following diseases and disorders: glycogen storage disease, cirrhosis due to liver, phosphorylase kinase deficiency, hepatitis, metabolic disorders, hypotonia malaria, and alcoholism. This protein has interactions with PHKG1, UBE3A, PHKA2, PHKB, and PYGL in PKA signaling, cAMP Pathway, activation of cAMP-dependent PKA, alpha-adrenergic signaling, metabolism of carbohydrates, glycogen breakdown (glycogenolysis), glucose metabolism, calcium signaling pathway and insulin signaling pathway.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: PHKG2
• Entrez:
  • 5261(Human)
• OMIM:
  • 172471(Human)
• Uniprot:
  • AAH02541(Human)