PHGDH Antibody

Images

 
Western Blot: PHGDH Antibody [NBP1-32201] - Wild-type (WT) and PHGDH knockout (KO) HeLa cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with PHGDH antibody [N1N2], N-term. ...read more
Immunocytochemistry/ Immunofluorescence: PHGDH Antibody [NBP1-32201] - Analysis of methanol-fixed HeLa, using PHGDH antibody at 1:500 dilution.
Immunohistochemistry-Paraffin: PHGDH Antibody [NBP1-32201] - Paraffin-embedded mouse pancreas. PHGDH antibody [N1N2], N-term diluted at 1:500.
Western Blot: PHGDH Antibody [NBP1-32201] - Sample (50 ug of whole cell lysate) A: Mouse brain 7. 5% SDS PAGE; antibody diluted at 1:1000.
Western Blot: PHGDH Antibody [NBP1-32201] - Sample(30 ug of whole cell lysate)A:293TB:A431 C:HeLa S3 7. 5% SDS PAGE, antibody diluted at 1:1000.
Immunohistochemistry-Paraffin: PHGDH Antibody [NBP1-32201] - Paraffin-embedded PC13 xenograft, using antibody at 1:500 dilution.
Immunoprecipitation: PHGDH Antibody [NBP1-32201] - Immunoprecipitation of PHGDH protein from 293T whole cell extracts using 5 ug of PHGDH antibody [N1N2], N-term (NBP1-32201). Western blot analysis was performed using ...read more

Product Details

Summary
Reactivity Hu, Mu, Rt, Po, Bv, PmSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-P, IP
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
0.87 mg/ml
     

Genetic Strategies

   

Order Details

PHGDH Antibody Summary

Immunogen
Recombinant protein encompassing a sequence within the N-terminus region of human PHGDH. The exact sequence is proprietary.
Predicted Species
Rat (97%), Porcine (97%), Bovine (97%), Primate (100%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
PHGDH
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:3000
  • Immunocytochemistry/Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Immunoprecipitation 1:100-1:500
Theoretical MW
57 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Expected cross reactivity based on sequence homology: Chimpanzee (100%).

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
0.1M Tris (pH 7.0), 0.1M Glycine and 10% Glycerol
Preservative
0.01% Thimerosal
Concentration
0.87 mg/ml
Purity
Immunogen affinity purified

Alternate Names for PHGDH Antibody

  • 3,3-phosphoglycerate dehydrogenase
  • 3PGDH
  • 3-PGDH
  • D-3-phosphoglycerate dehydrogenase
  • EC 1.1.1
  • EC 1.1.1.95
  • MGC3017
  • PDG
  • PGAD
  • PGD
  • PGDH
  • phosphoglycerate dehydrogenase
  • SERA

Background

3-Phosphoglycerate dehydrogenase (PHGDH; EC 1.1.1.95) catalyzes the transition of 3-phosphoglycerate into 3-phosphohydroxypyruvate, which is the first and rate-limiting step in the phosphorylated pathway of serine biosynthesis, using NAD+/NADH as a cofactor.[supplied by OMIM]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol PHGDH
Entrez
Uniprot