Home » PHACTR1 » PHACTR1 Antibodies » PHACTR1 Antibody

PHACTR1 Antibody

 (1 Publication)
 See All Related
Submit a Review

Images

 
(
Enlarge)
Western Blot: PHACTR1 Antibody [NBP1-56787] - Jurkat cell lysate, Antibody Titration: 0.2-1 ug/ml
Western Blot: PHACTR1 Antibody [NBP1-56787] - Jurkat cell lysate, Antibody Titration: 0.2-1 ug/ml
Immunohistochemistry-Paraffin: PHACTR1 Antibody [NBP1-56787] - Human Tonsil Tissue, 1ug/mL.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

100% Guarantee on every product

PHACTR1 Antibody Summary

Immunogen
Synthetic peptides corresponding to PHACTR1(phosphatase and actin regulator 1) The peptide sequence was selected from the middle region of PHACTR1. Peptide sequence QRPTAEELEQRNILKPRNEQEEQEEKREIKRRLTRKLSQRPTVEELRERK. The peptide sequence for this immunogen was taken from within the described region.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
PHACTR1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:10-1:500
  • Western Blot 1.0 ug/ml
Application Notes
This is a rabbit polyclonal antibody against PHACTR1 and was validated on Western blot.
Publications
Read Publication using
NBP1-56787 in the following applications:

  • WB
    1 publication

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
PBS and 2% Sucrose
Preservative
0.09% Sodium Azide
Purity
Immunogen affinity purified

Notes

The addition of 50% glycerol is optional for those storing this antibody at -20C and not aliquoting smaller units. However, please note that glycerol may interrupt some downstream antibody applications and should be added with caution.

Alternate Names for PHACTR1 Antibody

  • dJ257A7.2
  • KIAA1733MGC126577
  • MGC126575
  • phosphatase and actin regulator 1
  • RPEL repeat containing 1
  • RPEL
  • RPEL1

Background

PHACTR1 belongs to the phosphatase and actin regulator family. It contains 4 RPEL repeats. The function of the PHACTR1 protein remains unknown.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

Mkl1 Antibody
NBP1-88498
Immunohistochemistry-Paraffin: Mkl1 Antibody [NBP1-88498] - Staining of human liver shows very weak positivity in hepatocytes.
Immunohistochemistry-Paraffin: Mkl1 Antibody [NBP1-88498] - Staining of human lung shows moderate cytoplasmic positivity in macrophages.
Immunohistochemistry-Paraffin: Mkl1 Antibody [NBP1-88498] - Staining of human lymph node shows moderate positivity in lymphoid cells.
(7 Publications)
Species: Hu, Mu
Applications: ICC/IF, IHC, IHC-P, KD, WB
Serum Response
Factor SRF Antibody ( ...
NBP3-13785
Flow Cytometry: Serum Response Factor SRF Antibody (PCRP-SRF-1F7) [NBP3-13785] - Flow cytometric analysis of PFA-fixed HeLa cells. Serum Response Factor SRF antibody (PCRP-SRF-1F7) followed by goat anti-mouse IgG-CF488 (blue); isotype control (red).
Immunocytochemistry/Immunofluorescence: Serum Response Factor SRF Antibody (PCRP-SRF-1F7) [NBP3-13785] - Immunofluorescence Analysis of PFA-fixed HeLa cells stained using Serum Response Factor SRF Antibody (PCRP-SRF-1F7) followed by goat anti-mouse IgG-CF488 (green). CF640R phalloidin (red).
Immunohistochemistry-Paraffin: Serum Response Factor SRF Antibody (PCRP-SRF-1F7) [NBP3-13785] - IHC analysis of formalin-fixed, paraffin-embedded human colon. Strong nuclear staining using Serum Response Factor SRF antibody (PCRP-SRF-1F7) at 2ug/ml in PBS for 30min RT. HIER: Tris/EDTA, pH9.0, 45min. HRP-polymer, 30min. DAB, 5min.
Species: Hu
Applications: ELISA, Flow, ICC/IF, IHC-P, IP, PA, WB
CD8 Antibody
(53-6.7) - BSA Free
NBP1-49045
Immunohistochemistry-Paraffin: CD8 Antibody (53-6.7) [NBP1-49045] - CD8 alpha Antibody (53-6.7) [NBP1-49045] - CD8 alpha expression in mouse spleen tissue using anti-CD8 alpha antibody. Image from verified customer review.
Immunocytochemistry/Immunofluorescence: CD8 Antibody (53-6.7) [NBP1-49045] - CD8 alpha Antibody (53-6.7) [NBP1-49045] - Analysis of bone marrow tissue by single and multiple staining.
Immunohistochemistry-Frozen: CD8 Antibody (53-6.7) [NBP1-49045] - CD8 alpha Antibody (53-6.7) [NBP1-49045] - Analysis of mosue spleen sections. CD8+ lymphocytes are marked by brown labeling of the cell surface.
(3 Reviews)
(20 Publications)
Species: Mu, Rt
Applications: Cell Depl, CyTOF-ready, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP, InhibTFunc
TIRAP (TLR2 and
TLR4) Inhibitor Pepti ...
NBP2-26245
TIRAP (TLR2 and TLR4) Inhibitor Peptide [NBP2-26245] - TLR2 acts through formation of heterodimer complexes with TLR1 or TLR6. HEK 293 endogenously expresses TLR1 and TLR6, so that the TLR2 reporter cell line can response to both Pam3CSK4 (TLR2/TLR1 specific ligand) and MALP-2 (TLR2/TLR6 specific ligand). The TLR2 / TLR4 inhibitor specifically inhibits TLR2/TLR1 receptor complex activity in a dose-response manner but exhibited no or little effect on TLR2/TLR6 receptor complex activity.
TIRAP (TLR2 and TLR4) Inhibitor Peptide [NBP2-26245] - The TLR4 reporter cell line responds to LPS. The TLR2 / TLR4 inhibitor specifically inhibits TLR4 activation upon LPS stimulation in a dose-response manner.
TIRAP (TLR2 and TLR4) Inhibitor Peptide [NBP2-26245] - TLR2 /4 Inhibitor suppressed the Pam3CSK4-induced TLR2/TLR1 activity [A] and the LPS-induced TLR4 activity [B] in a dose-response manner, of which IC50 values were measured as 17.2 uM and 10.47 uM, respectively.
(6 Publications)
Species: Hu, Mu
Applications: Flow, In vitro
MAL Antibody
NBP2-22522
Western Blot: MAL Antibody [NBP2-22522] - Analysis of 50ug of mouse mpkCCD and Xenopus 2f3 whole cell lysates per well.
Species: Hu, Mu, Rt, Xp
Applications: WB
Myocardin Antibody
(355521)
MAB4028
Western blot shows lysates of MCF‑7 human breast cancer cell line, Raji human Burkitt's lymphoma cell line, HeLa human cervical epithelial carcinoma cell line, and C2C12 mouse myoblast cell line. PVDF membrane was probed with 1 µg/mL of Human Myocardin Monoclonal Antibody (Catalog # MAB4028) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for Myocardin at approximately 105 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
(11 Publications)
Species: Hu, Mu
Applications: WB
p19ARF/CDKN2A
Antibody - BSA Free
NB200-106
Immunohistochemistry: p19ARF/CDKN2A Antibody [NB200-106] - Adult mouse kidney (glomerulus). Image from verified customer review.
Western Blot: p19ARF/CDKN2A Antibody [NB200-106] - Analysis of p19ARF/CDKN2A in (A) NIH/3T3 and (B) MEF cells.
Immunocytochemistry/Immunofluorescence: p19ARF/CDKN2A Antibody [NB200-106] - Antibody was tested in MEF cells with DyLight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and DyLight 550 (red).
(1 Review)
(46 Publications)
Species: Mu, Rt
Applications: ELISA, ICC/IF, IHC, IHC-Fr, IHC-P, WB
Proprotein
Convertase 9/PCSK9 [H
DPC900
(45 Publications)
Species: Hu
Applications: ELISA
LDLR Antibody (C7)
NBP1-78159
Immunocytochemistry/Immunofluorescence: LDL R Antibody (C7) [NBP1-78159] - Antibody was tested in HepG2 cells with FITC (green). Nuclei were counterstained with DAPI (blue).
Immunohistochemistry: LDL R Antibody (C7) [NBP1-78159] - Analysis of an FFPE tissue section of human liver using LDL Receptor antibody (clone C7) with HRP-DAB based detection and hematoxylin counterstaining.
Immunocytochemistry/Immunofluorescence: LDL R Antibody (C7) [NBP1-78159] - Image from a customer review on porcine intestinal epithelial cells IPEC-J2.
(1 Review)
(22 Publications)
Species: Bv, Hu, Po, Rt
Applications: EM, ELISA, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP, In vitro, PLA, RIA, WB
TGF-beta RII
Antibody [Unconjugate
AF-241-NA
Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line, Hep3B human hepatocellular carcinoma cell line, U‑118‑MG human glioblastoma/astrocytoma cell line, HT1080 human fibrosarcoma cell line, and DU145 human prostate carcinoma cell line. PVDF membrane was probed with 2 µg/mL of Goat Anti-Human TGF‑ beta  RII Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-241-NA) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for TGF‑ beta  RII at approximately 75 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Recombinant Human TGF‑ beta 1 (Catalog # 240-B) inhibits Recombinant Human IL‑4 (Catalog # 204-IL) induced proliferation in the TF‑1 human erythroleukemic cell line in a dose-dependent manner (orange line). Inhibition of Recombinant Human IL‑4 (5 ng/mL) activity elicited by Recombinant Human TGF‑ beta 1 (0.04 ng/mL) is neutral­ized (green line) by increasing concentrations of Goat Anti- Human TGF‑ beta  RII Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-241-NA). The ND50 is typically10‑20 µg/mL.
TGF-beta  RII was detected in immersion fixed paraffin-embedded sections of human pituitary using Goat Anti-Human TGF-beta  RII Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-241-NA) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Lower panel shows a lack of labeling if primary antibodies are omitted and tissue is stained only with secondary antibody followed by incubation with detection reagents. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
(22 Publications)
Species: Hu
Applications: CyTOF-ready, ELISA(Cap), ELISA(Det), ELISA(Sta), Flow, IHC, Neut, WB
HNF1 Antibody
NBP1-33596
Western Blot: HNF1 Antibody [NBP1-33596] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with HNF1 alpha antibody [N1N3] diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.
Western Blot: HNF1 Antibody [NBP1-33596] - Analysis. Mouse tissue extracts (50 Ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with HNF1 alpha antibody [N1N3] diluted at 1:2000.
Western Blot: HNF1 Antibody [NBP1-33596] - Rat tissue extracts (50 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with HNF1 alpha antibody [N1N3] diluted at 1:1000.
(4 Publications)
Species: Hu, Mu, Rt
Applications: ICC/IF, IHC, IHC-P, IP, PAGE, WB
HSD17B4 Antibody
(OTI4C4)
NBP2-46005
Immunofluorescence: HSD17B4 Antibody (OTI4C4) [NBP2-46005] - Analysis of COS7 cells transiently transfected by pCMV6-ENTRY HSD17B4.
Immunofluorescence: HSD17B4 Antibody (OTI4C4) [NBP2-46005] - Analysis of HeLa cells.
Immunohistochemistry: HSD17B4 Antibody (OTI4C4) [NBP2-46005] - Analysis of Human Ovary tissue.
(1 Review)
(2 Publications)
Species: Hu, Mu, Rt
Applications: ICC/IF, IF, IHC, IHC-P, IP, PLA, WB
SOX4 Antibody
(CL5665)
NBP2-61421
Immunohistochemistry-Paraffin: SOX4 Antibody (CL5665) [NBP2-61421] - Staining of rat hippocampus shows strong nuclear immunoreactivity in a subset of cells in the granule cell layer of the dentate gyrus.
Immunohistochemistry-Paraffin: SOX4 Antibody (CL5665) [NBP2-61421] - Staining of human endometrium shows moderate to strong nuclear positivity in stromal cells.
Immunocytochemistry/Immunofluorescence: SOX4 Antibody (CL5665) [NBP2-61421] - Staining of SH-SY5Y cells using the Anti-SOX4 monoclonal antibody, showing specific staining in the nucleoplasm in green. Microtubule and nuclear probes are visualized in red and blue, respectively (where available).
Species: Hu, Mu, Rt
Applications: ICC/IF, IHC, IHC-P
C1D Antibody (6H2)
H00010438-M03
ELISA: C1D Antibody (6H2) [H00010438-M03] - Detection limit for recombinant GST tagged C1D is approximately 0.1ng/ml as a capture antibody.
Immunocytochemistry/Immunofluorescence: C1D Antibody (6H2) [H00010438-M03] - Analysis of monoclonal antibody to C1D on HeLa cell. Antibody concentration 10 ug/ml
Immunohistochemistry-Paraffin: C1D Antibody (6H2) [H00010438-M03] - Analysis of monoclonal antibody to C1D on formalin-fixed paraffin-embedded human colon. Antibody concentration 1 ug/ml
Species: Hu
Applications: ELISA, ICC/IF, IHC, IHC-P, WB
TCF21 Antibody
NBP1-88637
Immunohistochemistry-Paraffin: TCF21 Antibody [NBP1-88637] - Staining of human cerebral cortex shows no nuclear positivity in either neurons or glial cells as expected.
Immunohistochemistry-Paraffin: TCF21 Antibody [NBP1-88637] - Staining of human endometrium shows moderate nuclear positivity in a subset of glandular cells.
Immunohistochemistry-Paraffin: TCF21 Antibody [NBP1-88637] - Staining of human kidney shows moderate nuclear positivity in a subset of tubules as well as in some glomerular cells.
(3 Publications)
Species: Hu
Applications: ChIP, IHC, IHC-P, WB
Aurora A Antibody
- BSA Free
NBP1-51843
Western Blot: Aurora A Antibody [NBP1-51843] - WB detection of Aurora A in HeLa whole cell lysate.
Immunocytochemistry/Immunofluorescence: Aurora A Antibody [NBP1-51843] - HeLa cells were fixed and permeabilized for 10 minutes using -20C MeOH. The cells were incubated with anti- (NBP1-51843) at 2 ug/ml overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:1000 dilution for 60 minutes. Alpha tubulin (DM1A) NB100-690 was used as a co-stain at a 1:1000 dilution overnight at 4C and detected with an anti-mouse Dylight 550 (Red) at a 1:1000 dilution for 60 minutes. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 100X objective and digitally deconvolved.
Immunocytochemistry/Immunofluorescence: Aurora A Antibody [NBP1-51843] - IF Confocal analysis of HeLa cells using Aurora A antibody (NBP1-51843, 1:10). An Alexa Fluor 488-conjugated Goat to rabbit IgG was used as secondary antibody (green). Actin filaments were labeled with Alexa Fluor 568 phalloidin (red). DAPI was used to stain the cell nuclei (blue).
(1 Review)
(6 Publications)
Species: Hu, Mu
Applications: ICC/IF, IHC, IHC-P, Simple Western, WB
RhoD Antibody
NBP1-58359
Immunohistochemistry-Paraffin: RhoD Antibody [NBP1-58359] - Human Tonsil Tissue, 4-8ug/ml.
Western Blot: RhoD Antibody [NBP1-58359] - Transfected 293T, Antibody Titration: 0.2-1 ug/ml
Immunohistochemistry: RhoD Antibody [NBP1-58359] - Human Bronchial Epithelial Tissue Observed Staining: Cytoplasm and membrane of bronchial epithelial tissue Primary Antibody Concentration: 1 : 600 Secondary Antibody: Donkey anti-Rabbit-Cy3 Secondary Antibody Concentration: 1 : 200 Magnification: 20X.
Species: Hu
Applications: IHC, IHC-P, WB
PHACTR1 Antibody
NBP1-56787
PHACTR1 Antibody
Species: Hu
Applications: WB, IHC, IHC-P

Publications for PHACTR1 Antibody (NBP1-56787)(1)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 1 application: WB.


Filter By Application
WB
(1)
All Applications
Filter By Species
Human
(1)
All Species

Reviews for PHACTR1 Antibody (NBP1-56787) (0)

There are no reviews for PHACTR1 Antibody (NBP1-56787). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for PHACTR1 Antibody (NBP1-56787) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

 

Isotype Controls

Additional PHACTR1 Products

Bioinformatics Tool for PHACTR1 Antibody (NBP1-56787)

Discover related pathways, diseases and genes to PHACTR1 Antibody (NBP1-56787). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for PHACTR1 Antibody (NBP1-56787)

Discover more about diseases related to PHACTR1 Antibody (NBP1-56787).
 

Pathways for PHACTR1 Antibody (NBP1-56787)

View related products by pathway.

Blogs on PHACTR1

There are no specific blogs for PHACTR1, but you can read our latest blog posts.
Omicron Variant Antibodies

Customers Who Bought This Also Bought

Contact Information

Download our Western Blot eHandbook

Product PDFs

  SDS
  PDF Datasheet
  Request CofA

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our PHACTR1 Antibody and receive a gift card or discount.


Bioinformatics

Gene Symbol PHACTR1