PGRMC1 Antibody - BSA Free

Western Blot: PGRMC1 Antibody [NBP2-36525] - Samples: Whole cell lysate (50 ug) from HeLa, 293T, Jurkat, mouse TCMK-1, and mouse NIH3T3 cells prepared using NETN and RIPA lysis buffer. Antibodies: Affinity purified rabbit anti-PGRMC1 antibody used for WB at 0.4 ug/ml. Detection: Chemiluminescence with an exposuretime of 30 seconds.

Immunohistochemistry: PGRMC1 Antibody [NBP2-36525] - Sample: FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti- PGRMC1 used at a dilution of 1:5,000 (0.2ug/ml). Detection: DAB. Counterstain: IHC Hematoxylin (blue).

Immunoprecipitation: PGRMC1 Antibody [NBP2-36525] - Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from 293T cells prepared using RIPA lysis buffer. For blotting immunoprecipitated PGRMC1,it was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.

• Reactivity: Hu, Mu
• Applications: WB, IHC, IP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1.0 mg/ml

PGRMC1 Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit PGRMC1 Antibody - BSA Free (NBP2-36525) is a polyclonal antibody validated for use in IHC, WB and IP. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: The immunogen recognized by this antibody maps to a region between residue 145 to 195 of human Progesterone Receptor Membrane Component 1 using the numbering given in entry NP_006658.1.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: PGRMC1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry
  • Immunoprecipitation 2 - 10 ug/mg
  • Western Blot 1:1000 - 1:5000
• Application Notes: Antibody concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.
  Validation by IP/Western Blot was performed using a 4-12% Bis-Tris gel.

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: Tris-Citrate/Phosphate (pH 7.0 - 8.0)
• Preservative: 0.09% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for PGRMC1 Antibody - BSA Free

• HPR6.6
• HPR6.6PGRMC
• membrane-associated progesterone receptor component 1
• MPR
• PGRMC1
• Progesterone Binding Protein
• progesterone receptor membrane component 1

Background

Progesterone binding protein is a putative steroid membrane receptor. The protein is expressed predominantly in the liver and kidney.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: PGRMC1
• Uniprot:
  • NP_006658.1(Human)