PGLYRP1/PGRP-S Antibody (188C424) - Azide and BSA Free

Western Blot: PGLYRP1/PGRP-S Antibody (188C424) - Azide and BSA Free [NBP2-80909] - Analysis using PGLYRP1/PGRP-S antibody. Lysate from human Jurkat cells probed with PGRP-S antibody at 2 ug/ml. Image from the standard format of this antibody.

Immunohistochemistry: PGLYRP1/PGRP-S Antibody (188C424) - Azide and BSA Free [NBP2-80909] - IHC analysis of formalin fixed paraffin-embedded (FFPE) human esophagus using PGLYRPI/PGRP-S antibody at 20 ug/ml on a Bond Rx autostainer (Leica Biosystems). The assay involved 20 minutes of heat induced antigen retrieval (HIER) using 10mM sodium citrate

• Reactivity: Hu, Mu
• Applications: WB, Flow, ICC/IF, IHC, CyTOF-ready
• Clone: 188C424
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free
• Concentration: 1 mg/ml

PGLYRP1/PGRP-S Antibody (188C424) - Azide and BSA Free Summary

• Description: Novus Biologicals Mouse PGLYRP1/PGRP-S Antibody (188C424) - Azide and BSA Free (NB100-56719) is a monoclonal antibody validated for use in IHC, WB, Flow and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: This antibody was developed against KLH-conjugated synthetic peptide corresponding to amino acids 165-180 (YVLKGHRDVQRTLSPG) of human PGRP-S (NP_005082).
• Isotype: IgG3 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: PGLYRP1
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • Flow (Cell Surface) reported in scientific literature (PMID 15839897)
  • Flow (Intracellular) reported in scientific literature (PMID 19666596)
  • Flow Cytometry 1:20-1:20000
  • Immunocytochemistry/ Immunofluorescence 1:20-1:1000. Use reported in scientific literature (Dukhanina et al (2009))
  • Immunohistochemistry 1:20-1:1000
  • Immunohistochemistry-Frozen 1:20-1:1000. Use reported in scientific literature (Onkelen et al (2012))
  • Immunohistochemistry-Paraffin
  • Western Blot 2 ug/ml
• Application Notes: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• Theoretical MW: 21.73 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: No Preservative
• Concentration: 1 mg/ml
• Purity: Protein G purified

Alternate Names for PGLYRP1/PGRP-S Antibody (188C424) - Azide and BSA Free

• peptidoglycan recognition protein 1
• Peptidoglycan recognition protein short
• PGLYRP
• PGLYRP1
• PGRPpeptidoglycan recognition protein
• PGRPS
• PGRP-S
• PGRP-SMGC126894
• PGRPSMGC126896
• TAG7
• TNF superfamily, member 3 (LTB)-like (peptidoglycan recognition protein)
• TNFSF3L

Background

The primary immune recognition is based on structures common among invading pathogens. Bacterial surface molecules, such as lipopolysaccharide (LPS) and peptidoglycan (PGN), are known to elicit immune reactions ranging from cytokine release to fever. Recently, a family of proteins called peptidoglycan recognition protein (PGRP) has been identified in mouse and human that binds to peptidoglycans expressed on Gram-positive bacteria. Peptidoglycan (PGN) is an essential cell wall component of virtually all bacteria and, thus, it is an excellent target for recognition by the eukaryotic innate immune system. The PGRPs (PGRP-L, PGRP-S, PGRP-Ia, and PGRP-Ib) define a new family of human pattern recognition molecules. PGRP-L is primarily expressed in the liver. Although liver is not considered a primary immune organ, liver participates in host defenses by producing acute phase proteins (by hepatocytes) in response to infections and by clearing microorganisms from blood. PGRP-S is present in neutrophils and inhibits growth of Gram-positive bacteria and, therefore, may function as a neutrophil antibacterial protein. However, PGRP-S may have another, as yet unidentified function because in humans it is expressed in the bone marrow 50-100 times higher than in neutrophils.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: PGLYRP1