PGL2 Antibody - Azide and BSA Free

Western Blot: PGL2 Antibody [NBP2-94039] - Western blot analysis of extracts of various cell lines, using PGL2 antibody (NBP2-94039) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

• Reactivity: Hu, Mu
• Applications: WB
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: Azide and BSA Free

PGL2 Antibody - Azide and BSA Free Summary

• Description: Novus Biologicals Rabbit PGL2 Antibody - Azide and BSA Free (NBP2-94039) is a polyclonal antibody validated for use in WB. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-166 of human SDHAF2 (NP_060311.1). MAVSTVFSTSSLMLALSRHSLLSPLLSVTSFRRFYRGDSPTDSQKDMIEIPLPPWQERTDESIETKRARLLYESRKRGMLENCILLSLFAKEHLQHMTEKQLNLYDRLINEPSNDWDIYYWATEAKPAPEIFENEVMALLRDFAKNKNKEQRLRAPDLEYLFEKPR
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: SDHAF2
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • Western Blot 1:500 - 1:2000
• Theoretical MW: 19 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.3), 50% glycerol
• Preservative: 0.01% Thimerosal
• Purity: Affinity purified

Alternate Names for PGL2 Antibody - Azide and BSA Free

• C11orf79
• FLJ20487
• hSDH5
• paraganglioma or familial glomus tumors 2
• PGL2chromosome 11 open reading frame 79
• SDH5SDH assembly factor 2
• succinate dehydrogenase assembly factor 2, mitochondrial
• succinate dehydrogenase complex assembly factor 2
• Succinate dehydrogenase subunit 5, mitochondrial

Background

The SDHAF2 gene encodes a protein necessary for the flavination of the succinate dehydrogenase complex subunit SDHA (MIM 600857) and thus for SDH activity (Hao et al., 2009 (PubMed 19628817)).(supplied by OMIM)

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including Methotrexate, which is known to the State of California to cause reproductive toxicity with developmental effects. For more information, go to www.P65Warnings.ca.gov

Bioinformatics

• Gene Symbol: SDHAF2